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Basic data
| Standard ID | GB/T 31270.14-2025 (GB/T31270.14-2025) |
| Description (Translated English) | Test guidelines on environmental safety assessment for chemical pesticides - Part 14: Alga growth inhibition test |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B15 |
| Classification of International Standard | 65.100 |
| Word Count Estimation | 22,288 |
| Date of Issue | 2025-10-31 |
| Date of Implementation | 2026-05-01 |
| Older Standard (superseded by this standard) | GB/T 31270.14-2014 |
| Issuing agency(ies) | State Administration for Market Regulation and Standardization Administration of China |
GB/T 31270.14-2025: Test guidelines on environmental safety assessment for chemical pesticides - Part 14: Alga growth inhibition test
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.100
CCSB15
National Standards of the People's Republic of China
Replaces GB/T 31270.14-2014
Environmental Safety Evaluation Test Guidelines for Chemical Pesticides
Part 14.Algal Growth Inhibition Test
Published on 2025-10-31
Implemented on May 1, 2026
State Administration for Market Regulation
The State Administration for Standardization issued a statement.
Table of contents
Preface III
Introduction V
1.Scope 1
2 Normative References 1
3.Terms and Definitions 1
4.Principle 1
5.Test conditions 2
6.Reagents and Materials 2
7.Instruments and Equipment 3
8.Test and reference materials 3
9.Experimental Steps 4
10.Experimental Data Processing 5
11 Quality Control 6
12 Test Report 7
Appendix A (Informative) Preparation of Culture Media 9
Appendix B (Informative) Example 11.Calculation of the mean coefficient of variation for the mean specific growth rate of the control group
References 12
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents".
Drafting.
This document is Part 14 of GB/T 31270, "Test Guidelines for Environmental Safety Evaluation of Chemical Pesticides". GB/T 31270 has been published.
The following section.
---Part 1.Soil Metabolism Test;
---Part 2.Hydrolysis Test;
---Part 3.Photolysis Test;
---Part 4.Soil Adsorption/Desorption Tests;
---Part 5.Soil Leaching Test;
---Part 6.Volatility Testing;
---Part 7.Bioaccumulation Assays;
---Part 8.Metabolic Experiments of Water-Sediment Systems;
---Part 9.Short-term feeding toxicity tests in birds;
---Part 10.Acute toxicity tests on bees;
---Part 11.Acute Toxicity Tests on Silkworms;
---Part 12.Acute Toxicity Testing in Fish;
---Part 13.Acute Activity Inhibition Test in Daphnia;
---Part 14.Algal Growth Inhibition Test;
---Part 15.Acute toxicity test of earthworms;
---Part 16.Soil Microbial Toxicity Testing;
---Part 17.Acute toxicity test of the natural enemy Trichogramma wasp;
---Part 18.Acute toxicity testing in natural enemy amphibians;
---Part 19.Experiments on the Effects of Non-Target Plants;
---Part 20.Short-term feeding toxicity studies in livestock;
---Part 21.Toxicity Testing of Large Crustaceans;
---Part 22.Photolysis Test of Soil Surface;
---Part 23.Acute oral toxicity test in birds.
This document replaces GB/T 31270.14-2014 "Test Guidelines for Environmental Safety Evaluation of Chemical Pesticides Part 14.Algal Growth Inhibition".
Compared with GB/T 31270.14-2014, the main technical changes in the "Test" standard, apart from structural adjustments and editorial modifications, are as follows.
a) The scope has been changed (see Chapter 1, Chapter 1 of the.2014 edition);
b) The definitions of the terms "average specific growth rate" and "biomass increase" have been changed (see 3.2, 3.3; 2.2, 2.3 in the.2014 edition);
c) Six terms and definitions, including "test material," "chemical pesticide," "technical grade pesticide," "formulation," "active ingredient," and "reference material," have been deleted (see.2014).
(Year 2.4, 2.5, 2.6, 2.7, 2.8, 2.9)
d) The test conditions were changed (see Chapter 5, 4.1.5 of the.2014 edition);
e) Added relevant information about reagents (see 6.1);
f) The relevant information regarding the test organisms has been modified (see 6.2, 4.1.1 of the.2014 version);
g) Added relevant information about test containers (see 6.3);
h) The relevant content regarding the culture medium has been changed (see 6.4, 4.1.4 in the.2014 version);
i) The relevant content regarding instruments and equipment has been changed (see Chapter 7, 4.1.3 of the.2014 edition);
j) The relevant information regarding the test substance has been modified (see 8.1, 4.1.2 of the.2014 version);
k) The pre-culture method for algae solution has been modified (see 9.1, 4.2.1 of the.2014 edition);
l) Added relevant content on the preparation of the test drug solution (see 9.3.2);
m) The methods for exposure, culture, observation, and determination have been modified (see 9.3.3, 9.3.4, 9.3.5, and 4.2.3 of the.2014 edition);
n) A concentration detection method has been added (see 9.3.6);
o) Added relevant content on experimental data processing (see 10.1);
p) The statistical methods for experimental data have been changed (see 10.4, 4.3.3 of the.2014 version);
q) The quality control conditions have been changed (see Chapter 11, 4.4 of the.2014 edition);
r) The requirements for test reports have been changed (see Chapter 12, Chapter 5 of the.2014 edition);
s) The relevant content on the classification of pesticide toxicity levels to algae has been deleted (see Appendix B of the.2014 edition).
Please note that some content in this document may involve patents. The issuing organization of this document assumes no responsibility for identifying patents.
This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Technical Committee on Pesticide Standardization (SAC/TC133).
This document was drafted by the Institute for the Control of Agrochemicals, Ministry of Agriculture and Rural Affairs.
The main drafters of this document are. Chen Lang, Qu Mengmeng, Zhao Yu, Yang Hairong, Song Wencheng, Yuan Shankui, Wang Feidi, Yu Ruixian, Lan Shuai, and Zhou Binbin.
Pu Qianyun and Wu Chi.
The release history of this document and the document it replaces is as follows.
---First published in.2014 as GB/T 31270.14-2014;
---This is the first revision.
Introduction
To reduce the impact of pesticide use on the ecological environment and provide data that meets the requirements of pesticide environmental risk assessment, an environmental safety assessment is necessary.
The experiment aims to clarify the characteristics of pesticides in the environment, such as metabolism, degradation, adsorption, leaching, and bioaccumulation, as well as their toxicity to non-target organisms.
GB/T 31270, "Test Guidelines for Environmental Safety Evaluation of Chemical Pesticides," is a standard for test methods guiding environmental safety evaluation of pesticides in my country.
The standardization of pesticide environmental safety evaluation testing and the improvement of the scientific rigor and comparability of pesticide environmental safety evaluation testing consist of 23 parts.
---Part 1.Soil Metabolism Experiment. The aim is to clarify the metabolic pathways and rates of pesticides in soil.
---Part 2.Hydrolysis Tests. The purpose is to clarify the hydrolysis pathways and rates of pesticides.
---Part 3.Photolysis Experiment. The purpose is to clarify the photolysis pathway and rate of pesticides.
---Part 4.Soil Adsorption/Desorption Tests. The purpose is to clarify the soil adsorption characteristics of pesticides.
---Part 5.Soil Leaching Test. The purpose is to determine the leaching properties of pesticides.
---Part 6.Volatility Test. The purpose is to determine the volatility of pesticides.
---Part 7.Bioaccumulation Test. The purpose is to determine the bioaccumulation of pesticides in fish.
---Part 8.Metabolic Experiments in Aquatic-Sediment Systems. The aim is to clarify the metabolic pathways and metabolism of pesticides in aquatic-sediment systems.
Thanksgiving rate.
---Part 9.Short-term feeding toxicity studies in birds. The aim was to determine the short-term feeding toxicity of pesticides to birds.
---Part 10.Acute toxicity tests on bees. The purpose is to determine the acute toxicity of pesticides to bees.
---Part 11.Acute toxicity test on silkworms. The purpose is to determine the acute toxicity of pesticides to silkworms.
---Part 12.Acute Toxicity Tests in Fish. The purpose is to determine the acute toxicity of pesticides to fish.
---Part 13.Acute activity inhibition test of Daphnia. The purpose is to determine the acute toxicity of pesticides to Daphnia.
---Part 14.Algal Growth Inhibition Tests. The purpose was to determine the toxicity of pesticides to algae.
---Part 15.Acute toxicity test on earthworms. The purpose was to determine the acute toxicity of pesticides to earthworms.
---Part 16.Soil Microbial Toxicity Testing. The purpose is to determine the toxicity of pesticides to soil microorganisms.
---Part 17.Acute toxicity test of the natural enemy Trichogramma wasp. The purpose was to determine the toxicity of pesticides to Trichogramma wasps.
---Part 18.Acute toxicity tests on natural enemies (amphibians). The purpose is to determine the acute toxicity of pesticides to amphibians.
---Part 19.Effects on Non-Target Plants. The aim is to determine the toxicity of pesticides to non-target plants.
---Part 20.Short-term feeding toxicity studies in livestock. The aim is to determine the feeding toxicity of pesticides to livestock.
---Part 21.Toxicity Testing on Large Crustaceans. The purpose is to determine the toxicity of pesticides to large crustaceans.
---Part 22.Photodegradation Experiments on Soil Surfaces. The purpose is to clarify the photodegradation pathways and rates of pesticides on soil surfaces.
---Part 23.Acute oral toxicity tests in birds. The purpose is to determine the acute oral toxicity of pesticides to birds.
Environmental Safety Evaluation Test Guidelines for Chemical Pesticides
Part 14.Algal Growth Inhibition Test
Warning---Personnel using this document should have practical experience working in a formal laboratory. This document does not address all possible safety issues.
Users are responsible for taking appropriate safety and health measures and ensuring compliance with relevant national regulations.
1 Scope
This document describes the algal growth inhibition test method for environmental safety evaluation of chemical pesticides.
This document applies to algal growth inhibition tests conducted for the registration of chemical pesticides.
2 Normative references
The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included.
For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies.
This document.
NY/T 3273 Guidelines for Toxicity Testing of Difficult-to-Treat Pesticides on Aquatic Organisms
NY/T 4195.6 Cultivation of biological test materials for environmental impact testing of pesticide registration - Part 6.*Neptunus cephalopoda*
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
During a certain exposure period, compared with the control, the concentration of the test substance that caused an increase in the biomass of the tested algae or a 50% decrease in the average specific growth rate was...
Degree, expressed in EC50.
Note 1.In this document, EC50, calculated based on the inhibition rate of algal biomass growth, is represented by EyC50, which is calculated based on the inhibition rate of the average specific growth rate of algae.
The EC50 obtained by the rate calculation is denoted as ErC50.
Note 2.Units are mga.i./L and mg test substance/L.
3.2
Average specific growth rate during a specific exposure period.
Note. μ is the difference between the natural logarithm of the biomass of the tested algae at the end of the exposure and the beginning of the exposure, divided by the duration (e.g., 3 days).
3.3
biomass yield
Biomass growth during a specific exposure period.
Note. This refers to the biomass at the end of exposure minus the biomass at the beginning of exposure, denoted by Y.
4.Principles
A series of test solutions of different concentrations were prepared using the test substance and algal culture medium. After mixing the test solutions with the algal solutions, the subjects were continuously exposed to the solution.
...
