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Basic data
| Standard ID | GB/T 31270.10-2025 (GB/T31270.10-2025) |
| Description (Translated English) | Test guidelines on environmental safety assessment for chemical pesticides - Part 10: Honeybee acute toxicity test |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B13 |
| Classification of International Standard | 65.100 |
| Word Count Estimation | 22,283 |
| Date of Issue | 2025-10-05 |
| Date of Implementation | 2026-05-01 |
| Older Standard (superseded by this standard) | GB/T 31270.10-2014 |
| Issuing agency(ies) | State Administration for Market Regulation and Standardization Administration of China |
GB/T 31270.10-2025: Test guidelines on environmental safety assessment for chemical pesticides - Part 10: Honeybee acute toxicity test
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.100
CCSB15
National Standards of the People's Republic of China
Replaces GB/T 31270.10-2014
Environmental Safety Evaluation Test Guidelines for Chemical Pesticides
Part 10.Acute Toxicity Tests of Bees
Published on 2025-10-05
Implemented on May 1, 2026
State Administration for Market Regulation
The State Administration for Standardization issued a statement.
Table of contents
Preface III
Introduction V
1.Scope 1
2 Normative References 1
3.Terms and Definitions 1
4.Principle 2
5.Test conditions 2
6.Reagents and Materials 2
7.Instruments and Equipment 4
8.Test and reference materials 4
9.Experimental Steps 4
10.Experimental Data Processing 7
11 Quality Control 7
12 Test Report 8
Appendix A (Informative) Schematic diagram of queen bee egg-laying controller 10
Appendix B (Informative) Schematic diagram of a bee larvae rearing unit hole 11
Appendix C (Normative) Flowchart for Single Exposure Toxicity Test of Honeybee Larvae 12
Reference 13
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents".
Drafting.
This document is Part 10 of GB/T 31270, "Test Guidelines for Environmental Safety Evaluation of Chemical Pesticides". GB/T 31270 has been published.
The following section.
---Part 1.Soil Metabolism Test;
---Part 2.Hydrolysis Test;
---Part 3.Photolysis Experiment;
---Part 4.Soil Adsorption/Desorption Tests;
---Part 5.Soil Leaching Test;
---Part 6.Volatility Testing;
---Part 7.Bioaccumulation Assays;
---Part 8.Metabolic Experiments of Water-Sediment Systems;
---Part 9.Short-term feeding toxicity tests in birds;
---Part 10.Acute toxicity tests on bees;
---Part 11.Acute Toxicity Tests on Silkworms;
---Part 12.Acute Toxicity Testing in Fish;
---Part 13.Acute Activity Inhibition Test in Daphnia;
---Part 14.Algal Growth Inhibition Test;
---Part 15.Acute toxicity test of earthworms;
---Part 16.Soil Microbial Toxicity Testing;
---Part 17.Acute toxicity test of the natural enemy Trichogramma wasp;
---Part 18.Acute toxicity testing in natural enemy amphibians;
---Part 19.Experiments on the Effects of Non-Target Plants;
---Part 20.Short-term feeding toxicity studies in livestock;
---Part 21.Toxicity Testing of Large Crustaceans;
---Part 22.Photolysis Test of Soil Surface;
---Part 23.Acute oral toxicity test in birds.
This document replaces GB/T 31270.10-2014 "Test Guidelines for Environmental Safety Evaluation of Chemical Pesticides Part 10.Acute Toxicity of Honeybees".
Compared with GB/T 31270.10-2014, the main technical changes in the "Test" standard, apart from structural adjustments and editorial modifications, are as follows.
a) Definitions for bee larvae, adult worker bees, and brood comb have been added (see 3.2, 3.3, and 3.4);
b) The definitions of test substance, chemical pesticide, technical grade, formulation, active ingredient, and reference substance have been deleted (see sections 2.2 and 2.3 of the.2014 edition).
2.4, 2.5, 2.6, 2.7);
c) The basic procedures and objectives of single-exposure toxicity tests on bee larvae have been added (see 4.3);
d) The test conditions for single-exposure toxicity tests on bee larvae were increased (see 5.2);
e) The reagents and materials required for the experiment have been increased (see 6.1, 6.2);
f) Requirements have been added regarding the acquisition, feeding, composition, preparation, and storage of bee larvae (see 6.3.2 and 6.4);
g) The required instruments and equipment for the experiment have been increased (see 7.1, 7.2, and 7.6);
h) The requirements for test articles and reference articles have been changed (see 8.1, 8.2, and 4.1.2 of the.2014 edition);
i) The procedure for single-exposure toxicity testing of bee larvae has been improved (see 9.3);
j) The relevant descriptions of data processing have been revised, and the provisions for Koch's method, linear interpolation, and probability unit graphical method have been removed (see 10.1,
(2014 version 4.3);
k) Added data processing methods for single-exposure toxicity tests on bee larvae (see 10.2);
l) The quality control requirements have been changed (see 11.1, 4.4 of the.2014 edition);
m) Added quality control requirements for single-exposure toxicity tests of bee larvae (see 11.2);
n) The test report requirements have been changed (see 12.1, Chapter 5 of the.2014 edition);
o) Increased the reporting requirements for single-exposure toxicity tests on bee larvae (see 12.2);
p) The classification of acute toxicity levels of pesticides to bees has been removed (see Appendix A of the.2014 edition).
Please note that some content in this document may involve patents. The issuing organization of this document assumes no responsibility for identifying patents.
This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Technical Committee on Pesticide Standardization (SAC/TC133).
This document was drafted by the Institute for the Control of Agrochemicals, Ministry of Agriculture and Rural Affairs, and the Institute of Plant Protection, Chinese Academy of Agricultural Sciences.
The main drafters of this document are. Yuan Shankui, Zhu Lizhen, Wu Chi, Chen Lang, Zhou Yanming, Cang Tao, Bu Yuanqing, Liu Qiong, Zhao Changsheng, Zhang Haiqing, and Zhu Yuxuan.
Liao Jianhua.
The release history of this document and the document it replaces is as follows.
---First published in.2014 as GB/T 31270.10-2014;
---This is the first revision.
Introduction
To reduce the impact of pesticide use on the ecological environment and provide data that meets the requirements of pesticide environmental risk assessment, an environmental safety assessment is necessary.
The experiment aims to clarify the characteristics of pesticides in the environment, such as metabolism, degradation, adsorption, leaching, and bioaccumulation, as well as their toxicity to non-target organisms.
GB/T 31270, "Test Guidelines for Environmental Safety Evaluation of Chemical Pesticides," is a standard test method that guides pesticide environmental safety evaluation tests in my country.
The standard aims to regulate pesticide environmental safety evaluation testing and improve the scientific rigor and comparability of such tests. It consists of 23 parts.
constitute.
---Part 1.Soil Metabolism Experiment. The aim is to clarify the metabolic pathways and rates of pesticides in soil.
---Part 2.Hydrolysis Tests. The purpose is to clarify the hydrolysis pathways and rates of pesticides.
---Part 3.Photolysis Experiment. The purpose is to clarify the photolysis pathway and rate of pesticides.
---Part 4.Soil Adsorption/Desorption Tests. The purpose is to clarify the soil adsorption characteristics of pesticides.
---Part 5.Soil Leaching Test. The purpose is to determine the leaching properties of pesticides.
---Part 6.Volatility Test. The purpose is to determine the volatility of pesticides.
---Part 7.Bioaccumulation Test. The purpose is to determine the bioaccumulation of pesticides in fish.
---Part 8.Metabolic Experiments in Aquatic-Sediment Systems. The aim is to clarify the metabolic pathways and metabolism of pesticides in aquatic-sediment systems.
Thanksgiving rate.
---Part 9.Short-term feeding toxicity studies in birds. The aim was to determine the short-term feeding toxicity of pesticides to birds.
---Part 10.Acute toxicity tests on bees. The purpose is to determine the acute toxicity of pesticides to bees.
---Part 11.Acute toxicity test on silkworms. The purpose is to determine the acute toxicity of pesticides to silkworms.
---Part 12.Acute Toxicity Tests in Fish. The purpose is to determine the acute toxicity of pesticides to fish.
---Part 13.Acute activity inhibition test of Daphnia. The purpose is to determine the acute toxicity of pesticides to Daphnia.
---Part 14.Algal Growth Inhibition Tests. The purpose was to determine the toxicity of pesticides to algae.
---Part 15.Acute toxicity test on earthworms. The purpose was to determine the acute toxicity of pesticides to earthworms.
---Part 16.Soil Microbial Toxicity Testing. The purpose is to determine the toxicity of pesticides to soil microorganisms.
---Part 17.Acute toxicity test of the natural enemy Trichogramma wasp. The purpose was to determine the toxicity of pesticides to Trichogramma wasps.
---Part 18.Acute toxicity tests on natural enemies (amphibians). The purpose is to determine the acute toxicity of pesticides to amphibians.
---Part 19.Effects on Non-Target Plants. The aim is to determine the toxicity of pesticides to non-target plants.
---Part 20.Short-term feeding toxicity studies in livestock. The aim is to determine the feeding toxicity of pesticides to livestock.
---Part 21.Toxicity Testing on Large Crustaceans. The purpose is to determine the toxicity of pesticides to large crustaceans.
---Part 22.Photodegradation Experiments on Soil Surfaces. The purpose is to clarify the photodegradation pathways and rates of pesticides on soil surfaces.
---Part 23.Acute oral toxicity tests in birds. The purpose is to determine the acute oral toxicity of pesticides to birds.
Environmental Safety Evaluation Test Guidelines for Chemical Pesticides
Part 10.Acute Toxicity Tests of Bees
Warning---Personnel using this document should have practical experience working in a formal laboratory. This document does not address all possible safety issues.
Users are responsible for taking appropriate safety and health measures and ensuring compliance with relevant national regulations.
1 Scope
This document describes the acute toxicity test method for bees in the environmental safety evaluation of chemical pesticides.
This document applies to acute oral toxicity tests, acute contact toxicity tests, and honey toxicity tests conducted on adult worker bees for the registration of chemical pesticides.
Single-exposure toxicity test for bee larvae. This document does not apply to toxicity testing of volatile chemical pesticides.
2 Normative references
The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included.
For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies.
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
NY/T 4195.1 Environmental Impact Testing for Pesticide Registration - Biological Material Cultivation - Part 1.Honeybees
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Median lethal dose
In acute oral toxicity tests, acute contact toxicity tests, and single exposure toxicity tests of adult worker bees, it caused...
The dose of the test substance is when 50% of the test organisms die.
Note. LD50 is used to represent the value of bee or larva.
3.2
honeybee larva
The insect stage from hatching to pupation.
3.3
adult worker honeybee
Bees in a colony that possess the ability and behavior of field foraging.
3.4
broodcomb
A bee colony contains a large number of bee eggs, larvae of different ages, and pupae in its comb.
...
