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GB/T 29662-2013: Determination of kojic acid and kojic dipalmitate in cosmetics -- High performance liquid chromatography
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GB/T 29662-2013: Determination of kojic acid and kojic dipalmitate in cosmetics -- High performance liquid chromatography

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Determination of kojic acid and kojic dipalmitate in cosmetics. High performance liquid chromatography ICS 71.100.70 Y42 National Standards of People's Republic of China Determination of kojic acid in cosmetics, kojic acid palmitate High Performance Liquid Chromatography Issued on. 2013-09-06 2014-02-15 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard proposed by China National Light Industry Council. This standard by the National Standardization Technical Committee of Fragrance Flavor and Cosmetic (SAC/TC257) centralized. This standard was drafted. Shanghai Daily Chemical Industry Institute of Dalian City Product Quality Supervision, Inspection, Jahwa United shares have Limited. The main drafters of this standard. Cui Jian Jie, Li Qiong, Shen Min, Maoxi Qin, Hu Xia, Liu Chao, Xu Weidong. Determination of kojic acid in cosmetics, kojic acid palmitate High Performance Liquid Chromatography

1 Scope

This standard specifies the method for determination of kojic acid and its derivatives kojic dipalmitate cosmetics. This standard applies to the determination of kojic acid in cosmetics, and the detection limit was 0.01%, the limit of quantification of 0.1%. This standard applies to the determination of cosmetics kojic dipalmitate, and the detection limit was 0.04%, the limit of quantification of 0.4%.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis

3 Determination of Kojic Acid in Cosmetics

3.1 Principle Mobile phase - 1% aqueous acetic acid and methanol (10.90) as solvent, ultrasonic extraction, centrifugation, membrane filter of 0.45 m, the injection solution Equipped with a diode array detector (DAD) liquid chromatography detection, external standard. 3.2 Reagents and materials Unless otherwise specified, the reagents were of analytical grade. Water as a water GB/T 6682 regulations. 3.2.1 Methanol. chromatographically pure. 3.2.2 Kojic acid. purity of not less than 98%. 3.2.3 acetic acid. 3.2.4 1% aqueous solution of acetic acid. Take 10mL glacial acetic acid, dilute to 1000mL. 3.2.5 Mobile phase. methanol .1% acetic acid = 10.90. 3.2.6 Kojic acid standard stock solution. Weigh accurately 0.1g (accurate to 0.0001g) Kojic acid in 50mL beaker, add 1% aqueous acetic acid After the mixture was dissolved and transferred to 100mL volumetric flask, with a 1% aqueous solution of acetic acid to volume, that was kojic acid solution at a concentration of 1000mg/L Standard stock solution. 3-month storage seal shade. 3.2.7 standard solution. Pipette are standard stock solution (3.2.6) 0.2mL, 0.5mL, 1.0mL, 2.0mL, 5.0mL to 5 10mL volumetric flask, with the mobile phase (3.2.5) to the mark, formulated a series of concentrations of 0.02mg/mL, 0.05mg/mL, 0.1mg/mL, 0.2mg/L, 0.5mg/L of standard working solution. 3.3 instruments and equipment 3.3.1 High performance liquid chromatography with diode array detector. 3.3.2 micro injector, 10μL. 3.3.3 ultrasonic cleaner. ...