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Determination of urea content in cosmetics -- Enzymatic method
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Basic data
| Standard ID | GB/T 29661-2013 (GB/T29661-2013) |
| Description (Translated English) | Determination of urea content in cosmetics -- Enzymatic method |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | Y42 |
| Classification of International Standard | 71.100.70 |
| Word Count Estimation | 6,645 |
| Quoted Standard | GB/T 6682; GB/T 601 |
| Regulation (derived from) | National Standards Bulletin 2013 No. 17 |
| Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China |
| Summary | This standard specifies: cosmetics urea content Determination Enzymatic method. This standard applies to: cosmetics urea quantitative determination, their lower limit of quantification of 0. 025 %. |
GB/T 29661-2013: Determination of urea content in cosmetics -- Enzymatic method
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Determination of urea content in cosmetics. Enzymatic method
ICS 71.100.70
Y42
National Standards of People's Republic of China
Determination of urea content in cosmetics
Enzymatic method
Issued on. 2013-09-06
2014-02-15 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The standard proposed by China National Light Industry Council.
This standard by the National Standardization Technical Committee of Fragrance Flavor and Cosmetic (SAC/TC257) centralized.
This standard was drafted. Shanghai Daily Chemical Industry Institute (Fragrance Flavor and Cosmetic National Quality Supervision and Inspection Center), Dalian production
Product quality supervision and inspection.
The main drafters of this standard. Cui Jian Jie, Li Qiong, Lin Yan, Kang Wei, Sun Chih-Ching, Wu Haixia, Maoxi Qin.
Determination of urea content in cosmetics
Enzymatic method
1 Scope
This standard specifies the method for determination of urea in cosmetics content --- enzyme catalysis.
This standard applies to cosmetics quantitative determination of urea, the lower limit of quantitation of 0.025%.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
Preparation of GB/T 601 chemical reagent standard titration solution
Principle 3
Samples under acidic conditions, which contain urea urease is decomposed ammonium salt, with an acid titration, thereby calculating urea content.
CO (NH2) 2 2H2O →
Urease
(NH4) 2CO3
(NH4) 2CO3 2HCl = 2NH4Cl CO2 H2O
4 Reagents and materials
Unless otherwise specified, reagents were of analytical grade. Water for the three water GB/T 6682 regulations.
4.1 urease. 0.5g or less may be 0.25g urea at 40 ℃ ~ 45 ℃ completely decomposed within 1h. In accordance with the product instructions to save.
4.2 0.1mol/L hydrochloric acid. preparation methods according to GB/T 601.
4.3 0.1mol/LNaOH solution. Weigh 0.40gNaOH solid was dissolved in 100mL of water.
4.4 ethanol.
4.5 plastic film.
5. Apparatus
5.1-high beaker, 100mL.
5.2 Acid burette.
5.3 ultrasonic cleaning.
5.4 magnetic stirrer.
5.5 pH meter. accuracy 0.01.
5.6 Analysis of balance. a sense of the amount of 0.01g.
5.7 constant temperature water bath.
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