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Genuineness and purity verification of potato seed-tuber -- SSR molecular marker
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Basic data
| Standard ID | GB/T 28660-2012 (GB/T28660-2012) |
| Description (Translated English) | Genuineness and purity verification of potato seed-tuber -- SSR molecular marker |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B04 |
| Classification of International Standard | 65.020 |
| Word Count Estimation | 17,148 |
| Quoted Standard | GB 18133 |
| Regulation (derived from) | National Standards Bulletin No. 24 of 2012 |
| Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China |
| Summary | This standard specifies the SSR molecular markers of potato (Solanum tuberosum L.) varieties approach. This standard applies to potato authenticity and purity testing. |
GB/T 28660-2012: Genuineness and purity verification of potato seed-tuber -- SSR molecular marker
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Genuineness and purity verification of potato seed-tuber.SSR molecular marker
ICS 65.020
B04
National Standards of People's Republic of China
Seed Potato authenticity and purity identification
SSR markers
Issued on. 2012-09-03
2012-11-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee seed crops (SAC/TC37) centralized.
This standard was drafted. Tuber Crops Research Institute, Yunnan Normal University, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences, the Northeast Agricultural University agronomy
Institute, College of Horticulture, Huazhong Agricultural University, Heilongjiang Academy of Agricultural Sciences, Ministry of Agriculture Seed Potato Quality Supervision and Testing Center, the International
Potato Center (CIP) Beijing Liaison Office.
The main drafters of this standard. Li Canhui, Hao Dahai, Jin Liping, Yang Shizhong, pipe Zhaoxu, Huangsan Wen, Chen Yi, the Xie from China, Bai Yanju,
Guowei.
Seed Potato authenticity and purity identification
SSR markers
1 Scope
This standard specifies the method of SSR markers to identify potato (Solanumtuberosum L.) cultivars.
This standard applies to seed potatoes authenticity and purity testing.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 18133 virus-free potato seed
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Simple sequence repeat simplesequencerepeat; SSR
The basic unit of the genome of one to six nucleotides of the DNA sequence of repeated tandem configuration.
3.2
Polymerase chain reaction polymerasechainreaction; PCR
In the thermostable DNA polymerase, in a large number of in vitro rapid amplification of a specific DNA sequence-specific methods.
4 instruments and reagents
4.1 instruments and appliances
4.1.1 Gradient PCR instrument.
4.1.2 low-speed centrifuge.
4.1.3 electrophoresis (meet 3000V voltage) and supporting electrophoresis tank.
4.1.4 gel imaging analysis system.
4.1.5 refrigerator (-28 ℃ ~ 4 ℃).
4.1.6 UV/Vis spectrophotometer.
4.1.7 electric heated water bath (37 ℃, 42 ℃, 65 ℃ and 95 ℃).
4.1.8 ice machine.
4.1.9 Vortex.
4.1.10 autoclave.
4.1.11 liquid nitrogen tank.
4.1.12 acidity (pH meter).
4.1.13 Shaker.
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