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GB/T 27623.1-2011 PDF English

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GB/T 27623.1-2011: Pharmacodynamic test technical specification for aquaculture antimicrobial agent -- Part 1: Antibiotics susceptibility test of macro-broth dilution method
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Basic data

Standard ID GB/T 27623.1-2011 (GB/T27623.1-2011)
Description (Translated English) Pharmacodynamic test technical specification for aquaculture antimicrobial agent -- Part 1: Antibiotics susceptibility test of macro-broth dilution method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B50
Classification of International Standard 11.220
Word Count Estimation 9,929
Date of Issue 2011-12-30
Date of Implementation 2012-04-01
Quoted Standard GB/T 6682
Regulation (derived from) Announcement of Newly Approved National Standards No. 22 of 2011
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the use of antimicrobial agents in fish broth dilution method constants required for drug susceptibility testing reagents and materials, equipment, procedures, and quality control. This section applies to a constant fishing antimicrobial susceptibility tests broth dilution method.

GB/T 27623.1-2011: Pharmacodynamic test technical specification for aquaculture antimicrobial agent -- Part 1: Antibiotics susceptibility test of macro-broth dilution method





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Pharmacodynamic test technical specification for aquaculture antimicrobial agent Part 1. Antibiotics susceptibility test of macro-broth dilution method ICS 11.220 B50 National Standards of People's Republic of China Fishery antibacterial drugs efficacy test specification Part 1. Constant broth dilution method Drug susceptibility testing agent-Part 1. Antibioticssusceptibilitytestofmacro-brothdilutionmethod Issued on. 2011-12-30 2012-04-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

GB/T 27623 "fishing antimicrobial drug efficacy test specification" is divided into two parts. --- Part 1. Constant broth dilution susceptibility testing; --- Part 2. Artificial infection control experiment. This is Part 1 GB/T 27623 of. This section drafted in accordance with GB/T 1.1-2009 given rules. This section proposed by the People's Republic of China Ministry of Agriculture. This part of the National Standardization Technical Committee on Fisheries (SAC/TC156) centralized. This section drafted by. Chinese Academy of Fishery Sciences Yellow Sea Fisheries Research Institute. The main drafters of this section. Liu Qi, Li Jian, Wang Qun, wearing an aryl Yu. Fishery antibacterial drugs efficacy test specification Part 1. Constant broth dilution method Drug susceptibility testing

1 Scope

GB/T 27623 provisions of this part of the reagent and material constants broth dilution method Drug fishing antimicrobial agents susceptibility tests required Materials, equipment, operating procedures and quality control. This section applies to fishing antibacterials constant broth dilution susceptibility test.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis People's Republic of China Veterinary Pharmacopoeia (2010 edition) People's Republic of China Ministry of Agriculture Bulletin No. 1521

3 Reagents and materials

3.1 Chemicals Unless otherwise indicated, the section identified as analytical use of chemical reagents. 3.2 experimental water Unless otherwise indicated, shall comply with GB/T 6682 in the three water specifications. 3.3 Mueler-Hinton agar (MH agar) medium Product description by preparative plate dry medium, wherein MH agar medium in the test for Vibrio need to add 1.5% chlorine Sodium. 3.4 Mueler-Hinton broth (MH broth) medium By Description MH broth prepared with dry medium, wherein MH broth test for Vibrio need to add sodium chloride A final concentration of 1.5%. Each batch MH pH value of the broth at room temperature should be 7.2 to 7.4, should contain 20mg/L ~ 25mg/L Ca2 and 10mg/L ~ 12.5mg/LMg2. If using atomic absorption spectrometry methods such as Ca2 and Mg2 measured media content is not When enough media should add the appropriate amount of Ca2 and Mg2 sterile solution (Preparation of magnesium and calcium ions in solution see Appendix A); Tim Plus Ca2 and Mg2 of MH broth can not be re-autoclaved. 3.5 0.85% sterile saline Weigh 8.50g (accurate to 0.01g) of analytically pure sodium chloride, after adding 1L of distilled water were dissolved 121 ℃ 15min autoclaved. ...

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