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GB/T 26635-2025: Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents - High-performance liquid chromatography
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GB/T 26635-2025	English	374	Add to Cart	4 days [Need to translate]	Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents - High-performance liquid chromatography
GB/T 26635-2011	English	334	Add to Cart	3 days [Need to translate]	Animal and vegetable fats and oils -- Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography

GB/T 26635-2025: Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents - High-performance liquid chromatography

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 67.200.10 CCSX14 National Standards of the People's Republic of China Replaces GB/T 26635-2011 Tocopherols and Tocotrienols in Animal and Plant Oils Content determination by high performance liquid chromatography chromatography, IDT Released on December 31, 2025; to be implemented on July 1, 2026. State Administration for Market Regulation The State Administration for Standardization issued a statement.

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents". Drafting. This document replaces GB/T 26635-2011 "Determination of Tocopherols and Tocotrienols in Animal and Vegetable Oils by High Performance Liquid Chromatography". Compared with GB/T 26635-2011, the main technical changes in the "Law" are as follows, apart from structural adjustments and editorial modifications. ---The scope has been changed (see Chapter 1, Chapter 1 of the.2011 edition); ---The mobile phase has been changed (see 5.4,.2011 edition of 5.4); --- Replace n-hexane in the reagent with n-heptane (see 9.2.1,.2011 version 9.2.1). This document adopts ISO 9936.2016, "High-performance liquid chromatography for the determination of tocopherols and tocotrienols in animal and vegetable oils". quantity". The following minimal editorial changes have been made to this document. ---To align with existing standards, the standard name has been changed to "Determination of Tocopherols and Tocotrienols in Animal and Vegetable Oils by High Performance Liquid Chromatography". Chromatography ---Change the paragraphs in 6.1 to list items in sequence for easier reading and use. Please note that some content in this document may involve patents. The issuing organization of this document assumes no responsibility for identifying patents. This document was proposed by the National Food and Strategic Reserves Administration. This document is under the jurisdiction of the National Technical Committee on Standardization of Grain and Oil (SAC/TC270). This document was drafted by. COFCO Engineering Technology (Xi'an) International Engineering Co., Ltd., Henan Huace Testing Technology Co., Ltd., and China Grain Reserves Corporation Quality Inspection Center. Xin Co., Ltd., Shaanxi Liantong General Standard Technical Service Co., Ltd., Guizhou Provincial Forestry Survey and Planning Institute, Shaanxi Haisifu Biotechnology Co., Ltd. The company, Guomao Food Science Research Institute Co., Ltd., Guiyang University, National Food and Strategic Reserves Administration Research Institute, Wuhan University of Light Industry, Jiang Nanjing University, Henan University of Technology, Chongqing Youzhou Camellia Oil Technology Co., Ltd., Shaanxi Provincial Academy of Grain and Oil Sciences, COFCO Engineering Testing & Certification Co., Ltd. company. The main drafters of this document are. Ren Chunming, Li Junchao, Qin Xuelei, Shan Xiaoxue, Yang Hongmei, Gu Yanwen, Chang Yunhe, Li Shu, Wen Yu, and Wei Hanyu. Meng Yonghong, Liu Jia, Miao Yutian, Chen Xiuhong, Xue Yalin, Duan Zhangqun, He Dongping, Zhang Sihong, Wang Xingguo, Liu Yulan, Zhou Wanmeng, Liu Liang Hou Juncai, Feng Hongxia, Jiang Youjun, Luo Shilong, Jiang Min, Fan Yanni, Gong Shan, Wang Ping, Yang Qingbo. The release history of this document and the document it replaces is as follows. ---First published in.2011 as GB/T 26635-2011; ---This is the first revision. Tocopherols and Tocotrienols in Animal and Plant Oils Content determination by high performance liquid chromatography

1 Scope

This document describes the determination of free α-, β-, γ-, δ-tocopherols and tocotrienols in animal and vegetable oils using high-performance liquid chromatography (HPLC). Methods of measurement. This document applies to the determination of tocopherol and tocotrienol content in animal and vegetable oils. It is applicable to the determination of tocopherol and tocotrienol esters. The product is first subjected to saponification. This document does not apply to milk and dairy products (including fats derived from milk and dairy products). Note. A suitable method for low-temperature saponification is described in Appendix B.

2 Normative references

The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included. For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies. This document. ple) Note. GB/T 15687-2008 Preparation of animal and vegetable oil samples (ISO 661.2003, IDT)

3 Terms and Definitions

3.1 Tocopherol content The mass fraction of monomeric tocopherol determined using the methods specified in this document. Note. Content is expressed as an integer in milligrams per kilogram. 4.Principles The test sample was dissolved in n-heptane, and the monomers were separated by high-performance liquid chromatography (HPLC). The correction factor determined by the standard solution was used to calculate... Calculate the content of tocopherol and tocotrienol. 5.Reagents All reagents used were of chromatographic grade or equivalent. 5.1 Standard substances of α-, β-, γ- and δ-tocopherols and tocotrienols. When no tocopherol standard is available, a mixture of wheat germ oil and soybean oil is used to identify α-, β-, γ-, and δ-tocopherol. When tocotrienol standards are unavailable, palm oil can be used to identify α- and γ-tocotrienols, and the resulting chromatograms can be used to aid in identification. When testing the peaks in the chromatogram of a sample, it is advisable to use the corresponding calibration factor for tocopherol. ...