Path:
Home >
GB/T >
Page629 > GB/T 25168-2010
Price & Delivery
US$139.00 · In stock · Download in 9 secondsGB/T 25168-2010: Technical regulation of farm animals cDNA library construction and preservation
Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See
step-by-step procedureStatus: Valid
| Std ID | Version | USD | Buy | Deliver [PDF] in | Title (Description) |
| GB/T 25168-2010 | English | 139 |
Add to Cart
|
3 days [Need to translate]
|
Technical regulation of farm animals cDNA library construction and preservation
|
Click to Preview a similar PDF
Basic data
| Standard ID | GB/T 25168-2010 (GB/T25168-2010) |
| Description (Translated English) | Technical regulation of farm animals cDNA library construction and preservation |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B40 |
| Classification of International Standard | 65.020.30 |
| Word Count Estimation | 6,664 |
| Date of Issue | 2010-09-26 |
| Date of Implementation | 2011-03-01 |
| Quoted Standard | GB/T 6682 |
| Regulation (derived from) | National Standard Approval Announcement 2010 No.6 (Total No.161) |
| Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China |
| Summary | This standard specifies the animal cDNA library construction methods. This standard applies to pigs, cattle, sheep, horses, asses, chicken, ducks, geese and other poultry cDNA library construction and preservation. |
GB/T 25168-2010: Technical regulation of farm animals cDNA library construction and preservation
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Technical regulation of farm animals cDNA library construction and preservation
ICS 65.020.30
B40
National Standards of People's Republic of China
Livestock cDNA library construction and preservation of technical regulations
Issued on. 2010-09-26
2011-03-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee on Animal Husbandry (SAC/TC274) centralized.
This standard was drafted. Beijing Animal Husbandry and Veterinary Research Institute, Chinese Academy of Agricultural Sciences.
The main drafters of this standard. Mayue Hui, Wei Guan Jun, Lu Taofeng, Lixiang Chen, Tong Chun-ling, Lulu Yu, Liu Peng.
Livestock cDNA library construction and preservation of technical regulations
1 Scope
This standard specifies the livestock cDNA library construction methods.
This standard applies to pigs, cattle, sheep, horses, donkeys, chickens, ducks, geese and other poultry cDNA library construction and preservation.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
cDNA complementarydeoxyribonucleicacid
Mature mRNA as a template, under the action of reverse transcriptase synthesis of cDNA strand.
3.2
cDNA library cDNAlibrary
Total RNA will be in a particular organ or tissue specific developmental stage eukaryotic organisms extracted, through a series of biochemical reverse transcription, etc.
After the double-stranded cDNA was synthesized group, and then inserted into a suitable vector, the transformed host strains constitute a specific cDNA clone groups.
3.3
Oligonucleotide oligonucleotide
A class of short chain of nucleotides or less collectively only 20 base pairs, commonly used as a probe to determine the structure of DNA or RNA.
3.4
Plaque plaque
Was released phage infection of bacteria progeny phage, we will continue to infect surrounding cells, resulting in the growth of bacteria on agar plates moss
There are lysed, the transparent region is formed of a large number of cells.
4 Reagents and solution preparation
Unless otherwise specified, reagents were analytical grade or biochemical reagents. Test water meet the requirements of GB/T 6682 a water use.
4.1 diethylpyrocarbonate (diethypyrocarbonate, DEPC) treated water
In 1000mL of deionized water was added 1mLDEPC, 37 ℃ allowed to stand 12h, autoclaved (120 ℃ 30min).
4.2 2mol/LMgSO4
Weigh 246.50gMgSO4 · H2O, add 400mL double-distilled water (doubledistiledH2O, ddH2O) dissolved, set the volume to
500mL, autoclaving.
4.3 10mmol/LMgSO4
Measure 1mL2mol/LMgSO4 plus ddH2O constant volume to 200mL, autoclave, 30min.
4.4 1mol/LTris-Cl (pH7.5)
Weigh 121.14gTris, 800mLddH2O dissolved in alkali to adjust pH to 7.5, set the volume to 1L, autoclaved.
4.5 SM buffer
Weigh 5.80gNaCl, 2.00gMgSO4 · 7H2O, weighed 50mL1mol/LTris-Cl (pH7.5) and 5mL2% (mass
...
