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GB/T 23890-2009: Rapid determination of erucic acid and glucosinolate in rapeseed -- Photometry
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GB/T 23890-2009: Rapid determination of erucic acid and glucosinolate in rapeseed -- Photometry

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Rapid determination of erucic acid and glucosinolate in rapeseed. Photometry ICS 67.200.10 X14 National Standards of People's Republic of China Determination of the erucic acid rapeseed and glucosinolates spectrophotometry Posted 2009-05-26 2009-10-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard and Appendix B is an informative annex. This standard is proposed and managed by the People's Republic of China Ministry of Agriculture. This standard was drafted. Oil and the Ministry of Agriculture Products Quality Supervision and Testing Center, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences. The main drafters of this standard. Li Peiwu, Zhang, Jiangsu and Chongqing, Li Guangming, Wangxue Fang, Xie Lihua, Wang Youping. Determination of the erucic acid rapeseed and glucosinolates spectrophotometry

1 Scope

This standard specifies the erucic acid rapeseed, glucosinolate content determined by spectrophotometry. This standard applies to quick detection of canola seed and commercial seed erucic acid, glucosinolate content. The minimum detection limit of the method. 0.5% erucic acid, glucosinolate of 10μmol/g.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB 5491 grain and oilseeds testing for sampling, and sample method Laboratory use specifications and test methods GB/T 6682 Analysis

3 Terms and Definitions

The following terms and definitions apply to this standard. 3.1 Rapeseed oil contained a cis Δ13- docosenoic acid, the fatty acid composition of the share percentages are expressed. 3.2 Micromoles of glucosinolates contained rapeseed, called glucosinolates per gram or per gram of rapeseed meal in the total amount of sulfur glycoside FIG. Principle 4 4.1 Determination of erucic principle Erucic acid rapeseed oil is different, different turbidity in polyethylene glycol octylphenyl ether ethanol solution to form, according to the turbidity and erucic acid containing Determination of correlation between the amount of erucic acid. 4.2 Determination of glucosinolates principle Rapeseed glucosinolates Aspergillus oryzae glucosinolates hydrolase reacts with glucosinolates degradation products, and o-methyl aniline ethanol solution generated with special Intrinsic absorption of a colored product, the use of spectrophotometric determination of glucosinolate content.

5 Reagents

Unless otherwise specified, the reagents used were of analytical grade reagents. 5.1 Water, GB/T 6682, Grade 2. 5.2 ultra-pure water, GB/T 6682, level. 5.3 polyethylene glycol octylphenyl ether (C34H62O11) ethanol solution. 10mg/mL. Weigh 10.0g polyethylene glycol octylphenyl ether None Aqueous ethanol solution and dilute to 1000mL. 5.4 potassium dihydrogen sulfate (of KH2PO4) solution. 0.1mol/L. Weigh 1.36g potassium dihydrogen phosphate dissolved in distilled water and dilute to 100mL. ...