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GB/T 22332-2025: Diagnostic techniques for duck virus enteritis
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GB/T 22332-2025: Diagnostic techniques for duck virus enteritis

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ICS 11.220 CCSB41 National Standards of the People's Republic of China Replaces GB/T 22332-2008 Diagnostic techniques for duck viral enteritis Published on 2025-08-29 Implemented on 2026-03-01 State Administration for Market Regulation The State Administration for Standardization issued a statement.

Table of Contents

Preface III Introduction IV 1.Scope 1 2 Normative References 1 3.Terms and Definitions 1 4.Abbreviations 1 5.Clinical diagnosis 2 6.Sample Collection, Preservation and Processing 3 7.Virus isolation and identification 3 8.Neutralization test (fixed virus-dilution serum method) 5 9.Direct immunofluorescence 6. 10 Indirect ELISA 7 11 PCR 9 12.PCR for differentiating vaccine strains from wild-type strains 11. 13 Quantitative Real-Time PCR 12 14 Comprehensive Judgment 13 Appendix A (Normative) Solution Preparation 14 Appendix B (Informative) Primary Cell Preparation Methods 16 Appendix C (Informative) Method for Determining the Median Infectious Disease Digestion (TCID50) in Tissue Culture (Reed-Muench Method) 17 Appendix D (Informative) Schematic diagram of neutralization test sample addition 18 Appendix E (Informative) PCR and Quantitative Real-Time PCR Results (Figure 19)

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents". Drafting. This document replaces GB/T 22332-2008 "Diagnostic Techniques for Duck Viral Enteritis". Compared with GB/T 22332-2008, except for structural adjustments... Aside from integration and editorial changes, the main technical changes are as follows. ---Abbreviations have been added (see Chapter 4); ---The requirements for clinical diagnosis have been changed (see Chapter 5, Chapter 2 of the.2008 edition); ---The requirements for sample collection, preservation, and processing have been revised (see Chapter 6, Chapter 3 of the.2008 edition); ---The requirements for virus isolation and identification have been changed (see Chapter 7, Chapter 3 of the.2008 edition); ---A neutralization test has been added (see Chapter 8); ---Direct immunofluorescence assay has been added (see Chapter 9); ---An indirect ELISA method has been added (see Chapter 10); ---The requirements for PCR methods have been changed (see Chapter 11, Chapter 4 of the.2008 edition); ---Added a PCR method for differentiating vaccine strains from wild-type strains (see Chapter 12); ---Added a quantitative real-time PCR method (including methods for differentiating between vaccine strains and wild-type strains) (see Chapter 13); ---Added a comprehensive assessment of diagnostic results (see Chapter 14). Please note that some content in this document may involve patents. The issuing organization of this document assumes no responsibility for identifying patents. This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This document is under the jurisdiction of the National Technical Committee on Animal Health Standardization (SAC/TC181). This document was drafted by. China Animal Health and Epidemiology Center, Zhejiang Academy of Agricultural Sciences, and Poultry Research Institute of Shandong Academy of Agricultural Sciences. Institute, South China Agricultural University, Shandong Zhongxin Food Group Co., Ltd. The main drafters of this document are. Jiang Wenming, Liu Shuo, Chen Liu, Zhang Cun, Li Yufeng, Yun Tao, Cui Xuezhi, Wang Jingjing, Ye Weicheng, Yu Xiaohui, and Li Yang. Guo Xiaofeng and Liu Hualei. The release history of this document and the document it replaces is as follows. ---First published in.2008 as GB/T 22332-2008; ---This is the first revision.

introduction

Duck viral enteritis (DVE), also known as duck plague, is caused by herpesvirus A. Marek's virus (MDV) is an acute, febrile, and contagious disease of ducks, geese, and swans (order Anseriformes) caused by duck herpesvirus type 1 (DAVV1). The pathogen is duck herpesvirus type 1, also known as duck entereritis virus (DEV) or duck plague virus (DPV). And DVE virus (DVEV). This disease is characterized by high fever, vascular damage leading to tissue and body cavity bleeding, rash-like lesions of the digestive tract mucosa, and lymphatic organs. It is characterized by lesions and degenerative changes in parenchymal organs. This disease spreads rapidly, with high morbidity and mortality rates, seriously threatening the duck farming industry. The virus poses a potential threat to wild waterfowl. No other birds, mammals, or humans have been reported to be infected. The pathogen has only one serotype. Different strains of the virus vary in pathogenicity. Currently, prevention and control mainly rely on attenuated live vaccines. The diagnosis of this disease comprehensively considers clinical diagnosis, etiological diagnosis, serological diagnosis, and molecular biological diagnosis. (As a herpesviridae family) DEVs are prone to genome recombination and mutation; therefore, multiple target genes should be considered when differentiating between vaccine strains and wild-type strains for diagnosis. Line distinction. Diagnostic techniques for duck viral enteritis 1.Scope This document describes the clinical diagnosis and virus isolation and identification, neutralization test, direct immunofluorescence, and indirect enzyme-linked immunosorbent assay (ELISA) for duck viral enteritis. Laboratory diagnostic tests including ELISA, polymerase chain reaction (PCR), differential diagnosis between vaccine strains and wild-type strains using PCR, and quantitative real-time PCR are also included. Breakdown method. This document applies to the diagnosis, detection, monitoring, and epidemiological investigation of duck viral enteritis.

2 Normative references

The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included. For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies. This document. NY/T 541 Technical Specifications for Veterinary Diagnostic Sample Collection, Preservation and Transportation 3.Terms and Definitions This document does not contain any terms or definitions that need to be defined. 4.Abbreviations The following abbreviations apply to this document. CPE. Cytopathic Effect DEV. Duck Enteritis Virus DVE. Duck Viral Enteritis ...