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Determination of virginiamycin in feeds
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Determination of virginiamycin in feeds -- High performance liquid chromatography
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Basic data
| Standard ID | GB/T 22261-2025 (GB/T22261-2025) |
| Description (Translated English) | Determination of virginiamycin in feeds |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B46 |
| Classification of International Standard | 65.120 |
| Word Count Estimation | 12,197 |
| Date of Issue | 2025-06-30 |
| Date of Implementation | 2026-01-01 |
| Older Standard (superseded by this standard) | GB/T 22261-2008 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 22261-2025: Determination of virginiamycin in feeds
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT22261-2025
National Standard of the People's Republic of China
ICS 65.120CCS B 46
Determination of Virginiamycin in Feed
Released on June 30, 2025
2026-01-01 implementation
State Administration for Market Regulation
The National Standardization Administration issued
Replaces GB/T 22261-2008
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
This document replaces GB/T 22261-2008 "Determination of Virginiamycin in Feed - High Performance Liquid Chromatography" and is compatible with GB/T 22261-
Compared with.2008, in addition to structural adjustments and editorial changes, the main technical changes are as follows.
a) The scope of application, detection limit and quantification limit have been changed (see Chapter 1, Chapter 1 of the.2008 edition);
b) Liquid chromatography-tandem mass spectrometry has been added (see Chapter 4);
c) The principles, experimental procedures and experimental data processing of HPLC have been modified (see Chapter 5, Chapters 3 and 4 of the.2008 edition).
Chapter 7).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC 76).
This document was drafted by. Zhejiang Jindakang Animal Health Products Co., Ltd., Zhejiang Provincial Animal Disease Prevention and Control Center (Zhejiang Provincial Veterinary Drug and Feed
Materials Supervision Office).
The main drafters of this document are. Huang Juan, Qiu Chengjun, Liu Yan, Lv Weijun, Zhou Wei, Chen Jie, Chen Yong, Zhao Lili, Luo Chengjiang, Shen Hongxia, Yuan Lu,
Wang Bin, Zhang Zhijian, and Ruan Xin.
The previous versions of this document and the documents it replaces are as follows.
--First published in.2008 as GB/T 22261-2008;
--This is the first revision.
Determination of Virginiamycin in Feed
1 Scope
This document describes a method for the determination of virginiamycin in feed by liquid chromatography-tandem mass spectrometry and high performance liquid chromatography.
This document applies to the use of virginiana in compound feeds, concentrated feeds, concentrate supplements, additive premixes and mixed feed additives.
Determination of virginiamycin (calculated as the sum of virginiamycin M1 and virginiamycin S1).
The detection limit of the liquid chromatography-tandem mass spectrometry method in this document is 0.02 mg/kg, and the limit of quantification is 0.05 mg/kg; the detection limit of the high performance liquid chromatography method is 0.02 mg/kg, and the limit of quantification is 0.05 mg/kg.
The limit of detection is 0.5 mg/kg and the limit of quantification is 1 mg/kg.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 20195 Preparation of animal feed samples
3 Terms and Definitions
There are no terms or definitions that require definition in this document.
4 Liquid chromatography-tandem mass spectrometry
4.1 Principle
The analytes in the sample were extracted with ethyl acetate, purified by a purification salt package and solid phase extraction column, and determined by liquid chromatography-tandem mass spectrometry.
Standard quantitative method.
4.2 Reagents or materials
Unless otherwise specified, only analytical grade reagents were used.
4.2.1 Water. GB/T 6682, Grade 1.
4.2.2 Ethyl acetate.
4.2.3 n-Hexane.
4.2.4 Methanol. chromatographic grade.
4.2.5 Acetonitrile. chromatographic grade.
4.2.6 Formic acid. chromatographically pure.
4.2.7 Ammonium acetate solution (0.05 mmol/L, pH 4.0). Weigh 3.85 g of anhydrous ammonium acetate and dissolve it in water to make up to 1000 mL.
The pH was adjusted to 4.0 with glacial acetic acid.
4.2.8 Methanolic ammonium acetate solution. methanol (4.2.4) to ammonium acetate solution (4.2.7) = 1.3 (volume ratio).
4.2.9 40% methanol solution. Measure 40 mL of methanol (4.2.4), dilute to 100 mL with water, and mix thoroughly.
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