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GB/T 22260: Historical versions
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Determination of anabolic steroids in feeds - Liquid chromatography-tandem mass spectrometry
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| GB/T 22260-2008 | English | 319 |
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Determination of methyltestosterone in feeds -- High performance liquid chromatography tandem mass spectrometry
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Basic data
| Standard ID | GB/T 22260-2023 (GB/T22260-2023) |
| Description (Translated English) | Determination of anabolic steroids in feeds - Liquid chromatography-tandem mass spectrometry |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B46 |
| Classification of International Standard | 65.120 |
| Word Count Estimation | 14,171 |
| Date of Issue | 2023-08-06 |
| Date of Implementation | 2024-03-01 |
| Older Standard (superseded by this standard) | GB/T 22260-2008 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 22260-2023: Determination of anabolic steroids in feeds - Liquid chromatography-tandem mass spectrometry
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ICS 65.120
CCSB46
National Standards of People's Republic of China
Replace GB/T 22260-2008
Determination of protein anabolic hormones in feed
Liquid chromatography-tandem mass spectrometry
Published on 2023-08-06
2024-03-01 Implementation
State Administration for Market Regulation
Released by the National Standardization Administration Committee
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
This document replaces GB/T 22260-2008 "Determination of methyltestosterone in feed by high performance liquid chromatography tandem mass spectrometry" and is consistent with
Compared with GB/T 22260-2008, in addition to structural adjustments and editorial changes, the main technical changes are as follows.
a) Increased detection limit (see Chapter 1);
b) The principle has been changed (see Chapter 4, Chapter 3 of the.2008 edition);
c) Changed the extraction and purification of the sample (see 8.1, 8.2, 7.1, 7.2 of the.2008 edition);
d) The reference conditions for liquid chromatography and mass spectrometry have been changed (see 8.3.1 and 8.3.2, 7.3.1 and 7.3.2 of the.2008 edition);
e) Changed the qualitative and quantitative requirements for liquid chromatography-tandem mass spectrometry (see 8.3.4 and 8.3.5, 7.3.3 of the.2008 edition);
f) Changed the test data processing (see Chapter 9, 8.1 of the.2008 edition);
g) Changed precision (see Chapter 10, Chapter 9 of the.2008 edition).
Please note that some content in this document may be subject to patents. The publisher of this document assumes no responsibility for identifying patents.
This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76).
This document was drafted by. Shanghai Animal Disease Prevention and Control Center, Feed Research Institute of the Chinese Academy of Agricultural Sciences.
The main drafters of this document. Huang Shixin, Wang Bo, Gu Xu, Cao Ying, Zhang Jing, Yan Feng, Shang Jun, Wu Jianping, Jia Ming, Wang Jie, Li Junguo,
Zhang Wengang and Huang Jiaying.
The previous versions of this document and the documents it replaces are as follows.
---First published as GB/T 22260-2008 in.2008;
---This is the first revision.
Determination of protein anabolic hormones in feed
Liquid chromatography-tandem mass spectrometry
1 Scope
This document describes a method for the liquid chromatography-tandem mass spectrometry determination of protein anabolic hormones in feed.
This document is applicable to testosterone, methane and alpha in compound feeds, concentrated feeds, concentrate supplements, additive premixed feeds and mixed feed additives.
Basetestosterone, Boldirone, Meandrosterone, Androstenedione, Dehydroepiandrosterone, Nandrolone, Nandrolone Propionate, Stanozolol, Melengestrol Acetate, Progesterone
Determination.
The detection limit of methyltestosterone, stanozolol and melengestrol acetate in this document is 0.005mg/kg, and the detection limit of testosterone, bodiron, mestandrostenone and androstene is 0.005mg/kg.
The detection limit of diketone, dehydroepiandrosterone, nandrolone, nandrolone propionate, and progesterone is 0.02 mg/kg; methyltestosterone, stanozolol, and melengestrol acetate
The limit of quantification for ketones is 0.01mg/kg, and the determination of testosterone, bodiron, meandrosterone, androstenedione, dehydroepiandrosterone, nandrolone, nandrolone propionate, and progesterone.
The amount limit is 0.05mg/kg.
2 Normative reference documents
The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, the dated quotations
For undated referenced documents, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to
this document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 20195 Preparation of animal feed samples
3 Terms and definitions
There are no terms or definitions to be defined in this document.
4 Principles
The protein anabolic hormones in the sample were extracted with acetonitrile, purified by the adsorbent, and then detected with a liquid chromatography-tandem mass spectrometer using the internal standard method.
Quantitative.
5 reagents or materials
5.1 Water. GB/T 6682, Level 1.
5.2 Acetonitrile. chromatographically pure.
5.3 Formic acid. chromatographically pure.
5.4 50% acetonitrile solution. Take 50mL of acetonitrile (5.2), add water to dilute to 100mL, and mix well.
5.5 0.01% formic acid solution. Take 100μL of formic acid (5.3), add water to dilute to 1000mL, and mix well.
5.6 Standard stock solution (1mg/mL). Accurately weigh testosterone, methyltestosterone, bodiron, mestandrosterone, androstenedione, dehydroepiandrosterone, nodrol
0.01g each of nandrolone propionate, stanozolol, melengestrol acetate, and progesterone (accurate to 0.00001g) were placed in a 10mL brown container.
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