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| GB/T 22182-2025 | English | 214 |
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Determination of chlorophyll content of rapeseed - Spectrometric method
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| GB/T 22182-2008 | English | 154 |
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Rapeseed -- Determination of chlorophyll content -- Spectrometric method
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Basic data
| Standard ID | GB/T 22182-2025 (GB/T22182-2025) |
| Description (Translated English) | Determination of chlorophyll content of rapeseed - Spectrometric method |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | X14 |
| Classification of International Standard | 67.200.20 |
| Word Count Estimation | 10,182 |
| Date of Issue | 2025-12-31 |
| Date of Implementation | 2026-07-01 |
| Older Standard (superseded by this standard) | GB/T 22182-2008 |
| Issuing agency(ies) | State Administration for Market Regulation, Standardization Administration of China |
GB/T 22182-2025: Determination of chlorophyll content of rapeseed - Spectrometric method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 67.200.20
CCSX14
National Standards of the People's Republic of China
Replaces GB/T 22182-2008
Determination of chlorophyll content in rapeseed
Spectrometric method
Spectrometric method (IDT)
Published on 2025-12-31
Implemented on July 1, 2026
State Administration for Market Regulation
The State Administration for Standardization issued a statement.
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents".
Drafting.
This document supersedes GB/T 22182-2008 "Determination of Chlorophyll Content in Rapeseed - Spectrophotometric Method", and is consistent with GB/T 22182-2008.
In comparison, aside from structural adjustments and editorial changes, the main technical changes are as follows.
---The description of the micro mechanical grinder has been revised (see 6.3, 9.1, 9.2.1, and 6.3, 9.1, 9.2.1 in the.2008 edition);
---The units for the optical path length of the cuvettes have been changed (see Chapter 10, Chapter 10 in the.2008 edition);
---The repetitiveness requirement has been changed (see 11.2, 11.1 in the.2008 version);
---The reproducibility requirement has been changed (see 11.3, 11.2 in the.2008 edition).
This document adopts ISO 10519.2015 "Determination of chlorophyll content in rapeseed - Spectrophotometric method".
The following minimal editorial changes have been made to this document.
---To align with existing standards, the standard name has been changed to "Spectrophotometric Method for Determination of Chlorophyll Content in Rapeseed".
Please note that some content in this document may involve patents. The issuing organization of this document assumes no responsibility for identifying patents.
This document was proposed by the National Food and Strategic Reserves Administration.
This document is under the jurisdiction of the National Technical Committee on Standardization of Grain and Oil (SAC/TC270).
This document was drafted by. the Academy of Sciences of the National Food and Strategic Reserves Administration, the Standards and Quality Center of the National Food and Strategic Reserves Administration, and China...
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Nanjing University of Finance and Economics, Xinjiang Uygur Autonomous Region Grain and Oil Products Quality Supervision and Inspection Station, Inner Mongolia Autonomous Region
District Grain and Oil Standards and Quality Monitoring Center, Hubei Provincial Grain and Oil Food Quality Supervision and Testing Center, Yunnan Provincial Grain and Oil Science Research Institute (Yunnan Provincial Grain and Oil Production)
Product Quality Supervision and Testing Center), Huaiyin Institute of Technology.
The main drafters of this document are. Yang Qian, Yuan Qiang, Xue Yalin, Wei Fang, Xing Changrui, Wang Weiping, Chen Yanping, Sun Tinglin, Yang Jin, and Yang Rongling.
Duan Zhangqun.
The release history of this document and the document it replaces is as follows.
---First published in.2008 as GB/T 22182-2008;
---This is the first revision.
Determination of chlorophyll content in rapeseed
Spectrophotometry
1 Scope
This document describes a spectrophotometric method for determining the chlorophyll content of rapeseed.
This document applies to the determination of chlorophyll content in rapeseed.
This document is not applicable to the determination of chlorophyll content in oils and fats.
2 Normative references
The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included.
For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies.
This document.
mattercontent)
Note. GB/T 14489.1-2008 Determination of moisture and volatile matter content in oilseeds (ISO 665.2000, IDT)
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
chlorophyll content
Under the operating conditions specified in this document, the mass fraction of substances in the sample that can produce absorption bands at a wavelength of around 665 nm.
Note. Chlorophyll content is expressed in milligrams per kilogram (mg/kg).
4.Principles
In appropriate equipment, the sample is extracted with a prescribed extractant, and the absorbance of the sample extract solution is measured using a spectrophotometer to determine chlorophyll content.
The content of elements.
5.Reagents
Unless otherwise specified, all reagents were of analytical grade.
Take 100 mL of anhydrous ethanol into a 500 mL beaker, then add 300 mL of anhydrous isooctane (i.e., 2,2,5-trimethylpentane) or anhydrous...
n-Heptane or anhydrous petroleum ether (the basic components are C7 hydrocarbons, with a boiling range of 90℃~100℃).
...