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Determination of trypsin inhibitor activity of soya products
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Basic data
| Standard ID | GB/T 21498-2008 (GB/T21498-2008) |
| Description (Translated English) | Determination of trypsin inhibitor activity of soya products |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | X14 |
| Classification of International Standard | 67.200.20 |
| Word Count Estimation | 12,133 |
| Date of Issue | 2008-03-06 |
| Date of Implementation | 2008-08-01 |
| Quoted Standard | GB/T 6682 |
| Adopted Standard | ISO 14902-2001, IDT |
| Regulation (derived from) | State Health and Family Planning Commission Notice No. 11 of 2016 |
| Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China |
| Summary | This standard specifies the determination of soybean trypsin inhibitor activity. Trypsin inhibitor activity can be used to indicate the degree of roast soy products. This standard applies to the determination of soybean trypsin inhibitor activity. |
GB/T 21498-2008: Determination of trypsin inhibitor activity of soya products
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Determination of trypsin inhibitor activity of soya products
ICS 67.200.20
X14
National Standards of People's Republic of China
GB/T 21498-2008/ISO 14902.2001
Soybean trypsin inhibitor
Determination of Activity
(ISO 14902.2001, IDT)
Posted 2008-03-06
2008-08-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard is identical with ISO 14902.2001 "animal feed soybean trypsin inhibitor activity assay" (in English).
For ease of use, the standard ISO 14902.2001 includes the following editorial changes.
--- The "this International Standard" by "this standard";
--- Decimal by decimal points instead of the original text, "", ".";
--- Deleting the original standard name of "animal feed" the term used directly "soybean";
--- The Normative references in the "ISO 3696. 1987 for analytical laboratory use specifications and test methods" was changed to
"Laboratory use - Specification and test methods GB/T 6682 analysis";
--- On the forms increases the header;
--- The relevant formulas are numbered.
Appendix A of this standard is a normative appendix, Appendix B is an informative annex.
The standard proposed by the National Food Authority.
This standard by the National Standardization Technical Committee OILS.
This standard is drafted by. Nanjing University of Finance and Economics.
The main drafters of this standard. OILS, Yuan Jian, Wang Haifeng, Yang Xiaorong.
GB/T 21498-2008/ISO 14902.2001
Soybean trypsin inhibitor
Determination of Activity
1 Scope
This standard specifies the method for determination of soybean trypsin inhibitor activity. Trypsin inhibitor activity may be used to represent a large
Browning soy.
This standard applies to the determination of soybean trypsin inhibitor activity.
The method detection limit was 0.5mg/g.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
According to the procedures specified in the standard measure of the quality of trypsin inhibitor divided by the sample mass obtained.
NOTE. trypsin inhibitor activity in mg/g expressed.
Principle 4
Under pH9.5 conditions, trypsin inhibitor extracted from the sample. By addition of the substrate benzoyl -L- arginine - p-nitroanilide
(L-BAPA) measuring residual trypsin activity. The amount determined by spectrophotometry release of p-nitroaniline.
5 Reagents and materials
Use only analytical reagent.
5.1 Water. the lowest level 3, in line with GB/T 6682 requirements.
5.2 Sodium hydroxide solution. Ba (NaOH) = 0.01mol/L.
5.3 hydrochloric acid solution. Ba (HCl) = 6mol/L.
5.4 hydrochloric acid solution. Ba (HCl) = 1mol/L.
5.5 hydrochloric acid solution. Ba (HCl) = 0.1mol/L.
5.6 hydrochloric acid solution. Ba (HCl) = 0.001mol/L.
Acetic acid solution 5.7. Ba (CH3COOH) = 5.3mol/L.
5.8 calcium chloride (CaCl2 · 2H2O).
5.9 calcium chloride hydrochloric acid solution. Weigh 0.735g calcium chloride (5.8) was dissolved in 1L0.001mol/L hydrochloric acid solution (5.6), the pH is adjusted
To 3.0 ± 0.1.
GB/T 21498-2008/ISO 14902.2001
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