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GB/T 21107: Historical versions
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| GB/T 21107-2025 | English | 269 |
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Determination of horse and donkey-derived materials in feeds
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| GB/T 21107-2007 | English | 189 |
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Identification of horse and donkey derived materials in animal-originated feedstuffs -- PCR method
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Basic data
| Standard ID | GB/T 21107-2025 (GB/T21107-2025) |
| Description (Translated English) | Determination of horse and donkey-derived materials in feeds |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B46 |
| Classification of International Standard | 65.120 |
| Word Count Estimation | 13,160 |
| Date of Issue | 2025-06-30 |
| Date of Implementation | 2026-01-01 |
| Older Standard (superseded by this standard) | GB/T 21107-2007 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 21107-2025: Determination of horse and donkey-derived materials in feeds
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ICS 65.120
CCSB46
National Standard of the People's Republic of China
Replaces GB/T 21107-2007
Determination of horse and donkey derived ingredients in feed
Released on June 30, 2025
Implementation on January 1, 2026
State Administration for Market Regulation
The National Standardization Administration issued
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
This document replaces GB/T 21107-2007 "Qualitative detection method of horse and donkey derived ingredients in animal feed - PCR method"
Compared with GB/T 21107-2007, in addition to structural adjustments and editorial changes, the main technical changes are as follows.
a) The scope of application and detection limit have been changed (see Chapter 1, Chapter 1 of the.2007 edition);
b) Added a chapter on “Abbreviations” (see Chapter 4);
c) Added a chapter on “Real-time Fluorescence Polymerase Chain Reaction” (see Chapter 5);
d) The sample has been changed (see 6.4, Chapter 7 of the.2007 edition);
e) The result determination and presentation have been changed (see 6.6, Chapter 9 of the.2007 edition);
f) The laboratory pollution prevention and control measures have been modified (see Chapter 7, Chapter 10 of the.2007 edition);
g) Added a chapter on “Hazardous Waste Disposal” (see Chapter 8).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76).
This document was drafted by. Shanghai Customs Animal, Plant and Food Inspection and Quarantine Technical Center and New Hope Liuhe Co., Ltd.
The main drafters of this document are. Cai Yicun, Pan Liangwen, Han Wei, Li Yong, Xu Zhenjian, Yang Qing, Zhang Yuqiu, Zhang Chen, Shao Bingyu, Xue Junxin,
Lin Yingzheng.
The previous versions of this document and the documents it replaces are as follows.
--- GB/T 21107-2007, issued in.2007;
---This is the first revision.
Determination of horse and donkey derived ingredients in feed
1 Scope
This document describes methods for the detection of horse and donkey-derived ingredients in feed using real-time fluorescent polymerase chain reaction and polymerase chain reaction.
The real-time fluorescence PCR method in this document is applicable to compound feed, concentrated feed, concentrate supplement, feed raw materials, compound premix feed and
Qualitative detection of horse and donkey-derived ingredients in mixed feed additives; PCR method is applicable to the qualitative detection of horse and donkey-derived ingredients in animal-derived feed raw materials.
Detection.
The detection limit for this document is 0.1%.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 19495.3-2004 Methods for extraction and purification of nucleic acids for detection of genetically modified products
GB/T 20195 Preparation of animal feed samples
GB T27403-2008 Laboratory quality control specification for food molecular biology testing
3 Terms and Definitions
There are no terms or definitions that require definition in this document.
4 Abbreviations
The following abbreviations apply to this document.
bp. base pair
Ct value. cycle threshold
5 Real-time fluorescent polymerase chain reaction
5.1 Principle
Primers and probes were designed based on the specific DNA fragments of horses and donkeys, and real-time fluorescence PCR technology was used for amplification.
The fluorescent signal and Ct value generated in the reaction can be used to detect the DNA components derived from horses and donkeys.
...
