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GB/T 21101-2025: Determination of porcine-derived material in feeds
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GB/T 21101: Historical versions

Std IDVersionUSDBuyDeliver [PDF] inTitle (Description)
GB/T 21101-2025English249 Add to Cart 3 days [Need to translate] Determination of porcine-derived material in feeds
GB/T 21101-2007English189 Add to Cart 3 days [Need to translate] Identification of porcine derived materials in animal-originated feedstuffs -- PCR method

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Basic data

Standard ID GB/T 21101-2025 (GB/T21101-2025)
Description (Translated English) Determination of porcine-derived material in feeds
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B46
Classification of International Standard 65.120
Word Count Estimation 12,147
Date of Issue 2025-06-30
Date of Implementation 2026-01-01
Older Standard (superseded by this standard) GB/T 21101-2007
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 21101-2025: Determination of porcine-derived material in feeds


---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT21101-2025
ICS 65.120 CCSB46 National Standard of the People's Republic of China Replaces GB/T 21101-2007 Determination of porcine-derived ingredients in feed Released on June 30, 2025 Implementation on January 1, 2026 State Administration for Market Regulation The National Standardization Administration issued

Preface

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. This document replaces GB/T 21101-2007 "Qualitative detection method of porcine-derived ingredients in animal feed - PCR method" and Compared with GB/T 21101-2007, in addition to structural adjustments and editorial changes, the main technical changes are as follows. a) The scope of application and detection limit have been changed (see Chapter 1, Chapter 1 of the.2007 edition); b) The term and definition of “polymerase chain reaction” have been deleted (see 3.1 of the.2007 edition); c) Added abbreviations (see Chapter 4); d) Added real-time fluorescent polymerase chain reaction method (see Chapter 5); e) The sample has been changed (see 6.4, Chapter 7 of the.2007 edition); f) The result determination and presentation have been changed (see 6.6, Chapter 9 of the.2007 edition); g) The laboratory pollution prevention and control measures have been modified (see Chapter 7, Chapter 10 of the.2007 edition); h) Waste disposal has been modified (see Chapter 8, Chapter 11 of the.2007 edition). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76). This document was drafted by. Sichuan Well Testing Technology Co., Ltd., Chengdu Customs Technology Center, Chengdu Agricultural Science and Technology Vocational College, Qinghai Vocational College, Island Customs Technical Center, Shenzhen Customs Animal and Plant Inspection and Quarantine Technical Center, Dalian Customs Technical Center. The main drafters of this document are. Yang Miao, Yang Xinyi, Zhang Jing, Zhang Fengping, Lin Hua, Xiao Xian, Li Jianzhen, Lu Jiawen, Yu Xinghong, Zhang Yanhong, Lu Lihua, Lei Hui, Sun Tao, Ruan Zhouxi, Chen Xi, An Wei, Zheng Jing, Chen Yingqi, Xue Changhua, Zhang Qian, and Chen Runjiang. The previous versions of this document and the documents it replaces are as follows. ---First published in.2007 as GB/T 21101-2007; ---This is the first revision. Determination of porcine-derived ingredients in feed

1 Scope

This document describes the real-time fluorescent polymerase chain reaction (RT-PCR) and polymerase chain reaction (PCR) methods for the determination of porcine-derived ingredients in feed. (PCR) qualitative detection method. The real-time fluorescence PCR method in this document is applicable to compound feed, concentrated feed, concentrate supplement, compound premix feed, feed raw materials and mixed Qualitative detection of porcine-derived ingredients in compound feed additives. PCR method is suitable for the qualitative detection of porcine-derived ingredients in animal-derived feed raw materials. Detection. The detection limit for this document is 0.1%.

2 Normative references

The contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB/T 19495.3-2004 Methods for extraction and purification of nucleic acids for detection of genetically modified products GB/T 20195 Preparation of animal feed samples GB/T 27403-2008 Laboratory quality control specification for food molecular biology testing

3 Terms and Definitions

There are no terms or definitions that require definition in this document.

4 Abbreviations

The following abbreviations apply to this document. bp. base pair Ct. cycle threshold 5 Real-time fluorescence PCR 5.1 Principle Primers and probes were designed based on the specific sequence of porcine β-actin gene, and real-time fluorescence PCR was used for amplification. The fluorescent signal and Ct value generated in the reaction can realize the qualitative detection of porcine-derived components. ...

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