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Determination of β-receptor agonists in feeds - Liquid chromatography-tandem mass spectrometry
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Determination of ractopamine in animal feeds -- High performance liquid chromatographic method
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Basic data
| Standard ID | GB/T 20189-2025 (GB/T20189-2025) |
| Description (Translated English) | Determination of ��-receptor agonists in feeds - Liquid chromatography-tandem mass spectrometry |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B46 |
| Classification of International Standard | 65.120 |
| Word Count Estimation | 12,131 |
| Date of Issue | 2025-06-30 |
| Date of Implementation | 2026-01-01 |
| Older Standard (superseded by this standard) | GB/T 22147-2008, GB/T 20189-2006 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 20189-2025: Determination of β-receptor agonists in feeds - Liquid chromatography-tandem mass spectrometry
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National Standard of the People's Republic of China
ICS 65.120CCS B 46
Determination of β-receptor agonists in feed
Liquid chromatography-tandem mass spectrometry
Determination of β⁃receptor agonists in feeds-
Liquid chromatography⁃tandem mass spectrometry
Released on June 30, 2025
2026-01-01 implementation
State Administration for Market Regulation
The National Standardization Administration issued
Replaces GB/T 22147-2008, GB/T 20189-2006
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
This document replaces GB/T 20189-2006 "Determination of ractopamine in feed - High performance liquid chromatography method" and GB/T 22147-
2008 "Determination of salbutamol, ractopamine and clenbuterol hydrochloride in feed by liquid chromatography-mass spectrometry", GB/T 20189-
Compared with GB/T 22147-2008, in addition to structural adjustments and editorial changes, the main technical changes in.2006 are as follows.
a) The scope of application, detection limit and quantification limit have been changed, and 21 β-receptor agonists have been added as test objects (see Chapter 1, GB/T 20189-
2006 and Chapter 1 of GB/T 22147-2008);
b) The principles have been changed (see Chapter 4, Chapter 3 of GB/T 20189-2006 and GB/T 22147-2008);
c) The reagents or materials have been changed (see Chapter 5, Chapter 4 of GB/T 20189-2006 and GB/T 22147-2008);
d) The instruments and equipment have been changed (see Chapter 6, Chapter 5 of GB/T 20189-2006 and GB/T 22147-2008);
e) The sample preparation method has been changed and sampling has been deleted (see Chapter 7, GB/T 20189-2006 and GB/T 22147-2008).
Chapter 6);
f) The test procedures have been changed (see Chapter 8, 7.1 of GB/T 20189-2006 and 7.1.1 of GB/T 22147-2008);
g) The precision has been changed (see Chapter 10 of GB/T 20189-2006 and Chapter 9 of GB/T 22147-2008).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC 76).
This document was drafted by. Sichuan Provincial Feed Work Station, Sichuan Well Testing Technology Co., Ltd., Agricultural Quality Control Center of the Chinese Academy of Agricultural Sciences
Institute of Quality Standards and Testing Technology, Sichuan Agricultural Product Quality and Safety Center, Sichuan University.
The main drafters of this document are. Zhao Lijun, Zhang Fengping, Fan Xia, Wu Yashan, Deng Pingling, Liu Wenbin, Zhang Jing, Suo Decheng, Yue Qin, Li Yun,
Chen Hong, Ding Xiaoqiao, Li Qinrui, Wang Ying, Bian Zhengrong, and Chen Xiaochun.
The previous versions of this document and the documents it replaces are as follows.
--Published as GB/T 20189-2006 in.2006 and GB/T 22147-2008 in.2008;
--This is the first revision.
Determination of β-receptor agonists in feed
Liquid chromatography-tandem mass spectrometry
1 Scope
This document describes a method for the determination of 21 β-agonists in feed by liquid chromatography-tandem mass spectrometry.
This document applies to the use of bambuterol, fenoxypropionamide, carboplatin, and succinate in compound feeds, concentrates, concentrate supplements, and additive premixes.
Butrol, Clenpent, Clenpro, Clenserol, Clenbuterol, Ractopamine, Ritodrine, Clorprenaline, Mabutrol, Mapentol
Luo, hydroxymethyl clenbuterol, zilpaterol, salbutamol, terbutaline, tolbuterol, cibuterol, cimaterol, bromobuterol and bromo
Determination of Clenbuterol.
In this document, the detection limit of salbutamol, ractopamine, and clenbuterol was 5 μg/kg, and the quantification limit was 10 μg/kg; the
The detection limit was 10 μg/kg and the quantification limit was 50 μg/kg.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 20195 Preparation of animal feed samples
3 Terms and Definitions
There are no terms or definitions that require definition in this document.
4 Principles
The analytes in the sample were extracted with hydrochloric acid-methanol solution, purified by solid phase extraction column or filter membrane, and determined by liquid chromatography-tandem mass spectrometry.
Calibration was performed using a matching standard curve and quantification was performed using the external standard method.
5 Reagents or materials
Unless otherwise specified, only analytical grade reagents were used.
5.1 Water. GB/T 6682, Grade 1.
5.2 Methanol. chromatographic grade.
5.3 Acetonitrile. chromatographic grade.
5.4 Formic acid. chromatographic grade.
5.5 Hydrochloric acid solution (0.1 mol/L). Pipette 8.3 mL of hydrochloric acid, dilute with water to 1000 mL, and mix thoroughly.
5.6 Hydrochloric acid-methanol solution. Pipette 80 mL of hydrochloric acid solution (5.5), add 20 mL of methanol (5.2), and mix well.
5.7 5% ammonia methanol solution. Pipette 5 mL of ammonia and 95 mL of methanol (5.2) and mix well.
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