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Determination of ochratoxin A in feeds
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Basic data
| Standard ID | GB/T 19539-2025 (GB/T19539-2025) |
| Description (Translated English) | Determination of ochratoxin A in feeds |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B46 |
| Classification of International Standard | 65.120 |
| Word Count Estimation | 10,148 |
| Date of Issue | 2025-08-01 |
| Date of Implementation | 2026-02-01 |
| Older Standard (superseded by this standard) | GB/T 19539-2004 |
| Issuing agency(ies) | State Administration for Market Regulation, Standardization Administration of China |
GB/T 19539-2025: Determination of ochratoxin A in feeds
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT19539-2025
ICS 65.120
CCSB46
National Standard of the People's Republic of China
Replaces GB/T 19539-2004
Determination of ochratoxin A in feed
Released on August 1, 2025
Implementation on February 1, 2026
State Administration for Market Regulation
The National Standardization Administration issued
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
This document replaces GB/T 19539-2004 “Determination of ochratoxin A in feed”.
In addition to structural adjustments and editorial changes, the main technical changes are as follows.
a) The scope of application has been changed (see Chapter 1, Chapter 1 of the.2004 edition);
b) Thin layer chromatography (see Chapter 3 of the.2004 edition) has been deleted;
c) Enzyme-linked immunosorbent assay (ELISA) was deleted (see Chapter 4 of the.2004 edition);
d) Liquid chromatography-tandem mass spectrometry has been added (see Chapter 4).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76).
This document was drafted by. Guangzhou Huibiao Testing Technology Center and National Animal Husbandry Station.
The main drafters of this document are. Hao Yanjuan, Yang Yucheng, Zhao Enze, Wang Zhimin, Luo Zifeng, Su Shenglan, Liang Shuzhen, Lin Cuiwen, Luo Qun, Long Zhixi,
Shen Darong, Ding Zhenhao, Pan Huanyu, Xie Shuyue, and He Qixia.
The previous versions of this document and the documents it replaces are as follows.
---First issued in.2004 as GB/T 19539-2004;
---This is the first revision.
Determination of ochratoxin A in feed
1 Scope
This document describes a method for the determination of ochratoxin A in feed by liquid chromatography-tandem mass spectrometry.
This document applies to compound feeds, concentrated feeds, concentrate supplements, additive premixes, mixed feed additives and plant-based feeds.
Determination of ochratoxin A in raw materials.
The detection limit of this document is 0.2μg/kg and the quantification limit is 0.5μg/kg.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 20195 Preparation of animal feed samples
3 Terms and Definitions
There are no terms or definitions that require definition in this document.
4 Principles
Ochratoxin A in the sample was extracted with acetonitrile and water, cleaned up by immunoaffinity column, and detected by liquid chromatography-tandem mass spectrometry.
Prepare standard solution for calibration and use external standard method for quantification.
5 Reagents or materials
warn.
a) Any container that comes into contact with ochratoxin A must be immersed in a 4% sodium hypochlorite solution and cleaned after half a day for later use;
b) For safety reasons, analysts must wear medical latex gloves when operating.
Unless otherwise specified, only analytical grade reagents were used.
5.1 Water. GB/T 6682, Grade 1.
5.2 Methanol. Chromatographic grade.
5.3 Potassium hydroxide solution. Weigh 5.6 g of potassium hydroxide, dissolve it in water, and dilute to 100 mL with water.
5.4 Phosphate buffered saline (PBS). weigh 8.0 g sodium chloride, 1.2 g disodium hydrogen phosphate, 0.2 g potassium dihydrogen phosphate, and 0.2 g potassium chloride and dissolve them in
Add 900 mL of water, adjust the pH to 7.0 with potassium hydroxide solution (5.3), make up to 1 L with water, and mix well.
5.5 Extraction solution. Measure 800 mL of acetonitrile and.200 mL of water and mix well.
5.6 Eluent. Take 2 mL of acetic acid, dilute to 100 mL with methanol, and mix thoroughly.
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