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GB/T 17813: Historical versions
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Determination of nicotinic acid and folic acid in premix -- High performance liquid chromatography
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Determination of nicotinic acid and folic acid in compound premix. High-pressure liquid chromatography
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Basic data
| Standard ID | GB/T 17813-2018 (GB/T17813-2018) |
| Description (Translated English) | Determination of nicotinic acid and folic acid in premix -- High performance liquid chromatography |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B46 |
| Classification of International Standard | 65.120 |
| Word Count Estimation | 10,126 |
| Date of Issue | 2018-09-17 |
| Date of Implementation | 2019-04-01 |
| Older Standard (superseded by this standard) | GB/T 17813-1999 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 17813-2018: Determination of nicotinic acid and folic acid in premix -- High performance liquid chromatography
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(Determination of niacin and folic acid in additive premixed feeds - High performance liquid chromatography)
ICS 65.120
B46
National Standards of People's Republic of China
Replace GB/T 17813-1999
Determination of niacin and folic acid in additive premixed feed
High performance liquid chromatography
Published on.2018-09-17
Implementation of.2019-04-01
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard replaces GB/T 17813-1999 "Determination of Nicotinic Acid and Folic Acid in Composite Premixes by High Performance Liquid Chromatography".
Compared with GB/T 17813-1999, this standard is modified as follows except for editorial changes.
--- Revised the detection limit of the method and increased the limit of quantitation (see Chapter 1, Chapter 1 of the.1999 edition);
---Modified standard name;
--- Revised the principle of niacin and folic acid determination (see Chapters 3 and 4, Chapter 3 of the.1999 edition);
--- Modified the composition of the extract of niacin and folic acid (see Chapters 3 and 4, 4.6 of the.1999 edition);
--- Revised the mobile phase and chromatographic conditions of niacin and folic acid (see 3.2.7, 3.5.2 and 4.5.2, 7.2.1.1 of the.1999 edition);
--- Modified the preparation method of niacin standard solution (see 3.2.9, 4.5.1 of.1999 edition);
--- Compound premixed feed extraction method, increased use of disodium edetate dihydrate (EDTA) (see 3.5.1.2 and
4.5.1.2, 7.1) of the.1999 edition;
--- Added standard chromatogram of niacin and folic acid (see Appendix A).
This standard is proposed and managed by the National Feed Industry Standardization Technical Committee (SAC/TC76).
This standard was drafted. Institute of Agricultural Quality Standards and Testing Technology, Chinese Academy of Agricultural Sciences [National Feed Quality Supervision and Inspection Center
(Beijing)].
The main drafters of this standard. Li Lan, Jia Wei, Suo Decheng.
The previous versions of the standards replaced by this standard are.
---GB/T 17813-1999.
Determination of niacin and folic acid in additive premixed feed
High performance liquid chromatography
1 Scope
This standard specifies high performance liquid chromatography for the determination of niacin and folic acid in additive premixed feeds.
This standard applies to the determination of niacin and folic acid in vitamin premixed feed and compound premix feed.
The detection limit of niacin is 100mg/kg, the limit of quantification is 300mg/kg; the detection limit of folic acid is 15mg/kg, and the limit of quantification is 50mg/kg.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 14699.1 Feed sampling
Preparation of GB/T 20195 animal feed samples
3 Determination of niacin
3.1 Principle
The niacin in the sample was extracted with an acidic methanol aqueous solution, separated by high performance liquid chromatography, detected by ultraviolet light, and quantified by external standard method.
3.2 Reagents or solutions
Unless otherwise specified, the reagents used in this standard are of analytical grade, and the water meets the requirements of Class I water in GB/T 6682.
3.2.1 Glacial acetic acid. excellent grade pure.
3.2.2 Sodium heptane sulfonate. chromatographically pure.
3.2.3 Triethylamine. excellent grade pure.
3.2.4 Methanol. chromatographically pure.
3.2.5 0.1% trifluoroacetic acid solution. Pipette 1 mL of trifluoroacetic acid in 1000 mL of water.
3.2.6 Extract. Weigh 50mg of disodium edetate dihydrate (hereinafter referred to as EDTA) dissolved in about 800mL of water, add
20 mL of glacial acetic acid (3.2.1) and 5 mL of triethylamine (3.2.3) were mixed and mixed with.200 mL of methanol. The pH of the solution was about 3-4.
3.2.7 Mobile phase. Weigh 1.1 g of sodium heptane sulfonate (3.2.2), 50 mg of EDTA dissolved in about 1000 mL of water, and add 20 mL of glacial acetic acid.
(3.2.1), 5 mL of triethylamine (3.2.3), mix, adjust the pH of the solution to 4.0 with glacial acetic acid and triethylamine, and pass the 0.45 μm filter. Take the above
800 mL of the solution was mixed with.200 mL of methanol (3.2.4) for use.
3.2.8 Niacin standard. niacin content ≥ 98.0%.
3.2.9 Niacin Standard Stock Solution. Accurately weigh 0.1g (accurate to 0.0001g) niacin standard (3.2.8) and place it in 100mL brown volume.
In a measuring flask, add water to dissolve it, and add 1 mL of 0.1% trifluoroacetic acid solution (3.2.5), dilute to the mark with water, and shake well. The standard reserve
The niacin content in the liquid is about 1mg/mL, and it is stored at 2°C~8°C, and the effective period is 6 months.
3.2.10 Niacin Standard Working Fluid. Adjust the dilution factor according to the type of sample (vitamin premix feed, compound premix feed) to make the standard
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