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Forensic sciences - Examination methods for carboxyhaemoglobin in blood samples - Spectrophotometry
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Basic data
| Standard ID | GA/T 2073-2023 (GA/T2073-2023) |
| Description (Translated English) | Forensic sciences - Examination methods for carboxyhaemoglobin in blood samples - Spectrophotometry |
| Sector / Industry | Public Security (Police) Industry Standard (Recommended) |
| Classification of Chinese Standard | A92 |
| Classification of International Standard | 13.310 |
| Word Count Estimation | 12,162 |
| Date of Issue | 2023-03-01 |
| Date of Implementation | 2023-12-01 |
| Issuing agency(ies) | Ministry of Public Security |
GA/T 2073-2023: Forensic sciences - Examination methods for carboxyhaemoglobin in blood samples - Spectrophotometry
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 13.310
CCSA92
Public safety industry standards of the People's Republic of China
Forensic Science Blood Carboxyhemoglobin Test
Implemented on 2023-12-01
Released by the Ministry of Public Security of the People's Republic of China
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some content in this document may be subject to patents. The publisher of this document assumes no responsibility for identifying patents.
This document is submitted by the Criminal Investigation Bureau of the Ministry of Public Security.
This document is under the jurisdiction of the Toxicology Analysis Sub-Technical Committee of the National Technical Committee for Standardization of Criminal Technology (SAC/TC179/SC1).
This document was drafted by. Ministry of Public Security Identification Center, Beijing Municipal Public Security Bureau, Hunan Provincial Public Security Department, Shanxi Provincial Public Security Department, Guangdong Provincial Public Security Department, Chengdu
Metropolitan Public Security Bureau, Inner Mongolia Autonomous Region Public Security Department, Dongguan Municipal Public Security Bureau, Guiyang Municipal Public Security Forensic Authentication Center, Zunyi Municipal Public Security Forensic Authentication Center
Center, Bijie City Public Security Forensic Appraisal Center.
The main drafters of this document. Wang Ruihua, Wei Chunming, Zhang Yunfeng, Chang Jing, Li Jiayi, Qiao Jing, Yan Xiaojun, Zhang Gaoqin, Pei Maoqing, Zhang Liang,
Huang Sicheng, Li Yan, Zhang Qiang, Zhang Lei, Wang Yi, Yang Lunkun, Xiang Hai, and Du Song.
Forensic Science Blood Carboxyhemoglobin Test
Spectrophotometry
1 Scope
This document specifies the spectrophotometric test method for carboxyhemoglobin saturation (COHb%) in blood for forensic science.
This document is suitable for the quantitative analysis of carboxyhemoglobin saturation in blood for forensic science.
2 Normative reference documents
The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, the dated quotations
For undated referenced documents, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to
this document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GA/T 122 Toxicological analysis terminology
3 Terms and definitions
The terms and definitions defined in GA/T 122 apply to this document.
4 Principles
After carbon monoxide enters the body, it combines with hemoglobin to form carboxyhemoglobin. The hemoglobin in the blood reduces hemoglobin and oxygenates hemoglobin.
It exists in four forms. protein, carboxyhemoglobin and methemoglobin. Different hemoglobins have different absorption spectra under ultraviolet and visible light.
Under the action of the reducing agent, oxyhemoglobin and methemoglobin are reduced to reduced hemoglobin, while carboxyhemoglobin is not reduced and remains.
Hemoglobin in the original blood exists in two forms. reduced hemoglobin and carboxyhemoglobin. The different absorption characteristics of these two substances are used to
The properties and absorbance values were tested according to Lambert-Beer's law.
Use the blank sample and the added sample as a control, and according to the requirements of parallel operations, after processing the blood, use spectrophotometry to conduct
detection.
5 Reagents and materials
5.1 Reagents
The experimental water should comply with the third-grade water specified in GB/T 6682.The reagents used in the analysis are all of analytical grade or above. The reagents include.
a) Sodium dithionite (Na2S2O4);
b) Concentrated ammonia (NH3·H2O). mass fraction 25%~28%;
c) 1-octanol;
d) Reducing diluent (0.4% ammonia solution containing 1.0% Na2S2O4). Accurately weigh 1.0g of sodium dithionite and dissolve in water, add
Add 0.4 mL of concentrated ammonia water, adjust the volume to 100 mL, mix well, and prepare immediately;
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