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Forensic sciences - Examination methods for five Amanita toxins including α-amanitin in biological samples - LC-MS
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Basic data
| Standard ID | GA/T 2070-2023 (GA/T2070-2023) |
| Description (Translated English) | Forensic sciences - Examination methods for five Amanita toxins including ��-amanitin in biological samples - LC-MS |
| Sector / Industry | Public Security (Police) Industry Standard (Recommended) |
| Classification of Chinese Standard | A92 |
| Classification of International Standard | 13.310 |
| Word Count Estimation | 14,130 |
| Date of Issue | 2023-03-01 |
| Date of Implementation | 2023-12-01 |
| Issuing agency(ies) | Ministry of Public Security |
GA/T 2070-2023: Forensic sciences - Examination methods for five Amanita toxins including α-amanitin in biological samples - LC-MS
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ICS 13.310
CCSA92
Public safety industry standards of the People's Republic of China
α-amanitin, etc. in forensic science biological examination materials
Detection of 5 Amanita Toxins by Liquid Chromatography-Mass Spectrometry
Published on 2023-03-01
Implemented on 2023-12-01
Released by the Ministry of Public Security of the People's Republic of China
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some content in this document may be subject to patents. The publisher of this document assumes no responsibility for identifying patents.
This document is proposed and submitted by the Toxic Analysis Sub-Technical Committee of the National Technical Committee for Standardization of Criminal Technology (SAC/TC179/SC1).
Return to home.
This document was drafted by. Ministry of Public Security Identification Center, China Criminal Police College, Zhejiang Police College.
The main drafters of this document. Chang Jing, Ren Xinxin, Zou Bo, Zhao Peng, Chen Xueguo, Wang Aihua, Wang Ruihua, Li Jiayi, Zhang Yunfeng, Yao Weixuan,
Huang Jian, Wang Fanglin, Yu Zhongshan.
α-amanitin, etc. in forensic science biological examination materials
Detection of 5 Amanita Toxins by Liquid Chromatography-Mass Spectrometry
1 Scope
This document stipulates α-amanitin, β-amanitin, and γ-amanitin in biological test materials (blood, urine, liver, etc.) in the field of forensic science.
Liquid chromatography-mass spectrometry (LC-MS) detection method for five kinds of amanitaxins including peptide, carboxydihydroxyphallulin, and dihydroxyphallulin.
This document is applicable to α-amanitin, β-amanitin, and γ-amanitin in biological test materials (blood, urine, liver, etc.) in the field of forensic science.
Qualitative and quantitative analysis of five kinds of amanitaxins including peptide, carboxydihydroxyphallulin, and dihydroxyphallulin. Other inspection materials can refer to
use.
2 Normative reference documents
The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, the dated quotations
For undated referenced documents, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to
this document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GA/T 122 Toxicological analysis terminology
3 Terms and definitions
The terms and definitions defined in GA/T 122 apply to this document.
4 Principles
Using blank samples and added samples as controls, according to the requirements of parallel operations, five kinds of Amanita toxins were tested on biological test materials (blood, urine, liver, etc.)
Extract, purify and concentrate compounds (see Appendix A for basic compound information). Liquid chromatography-mass spectrometry was used for detection, with retention time and mass spectrometry characteristics
The ion pair and ion pair abundance ratio are used as the basis for qualitative judgment; the chromatographic peak area is used as the basis, and the external standard single-point calibration method is used for quantification.
analyze.
5 Reagents and materials
5.1 Reagents
The experimental water should comply with the first-class water specified in GB/T 6682.Unless otherwise stated, the reagents used in the analysis are of chromatographic grade.
Reagents include.
a) Acetonitrile;
b) 10% methanol solution (take the preparation of 100mL as an example). measure 10mL methanol and 90mL water, and mix;
c) 1% formic acid acetonitrile solution (take preparation of 100mL as an example). measure 1mL formic acid and 99mL acetonitrile and mix evenly;
d) 5% methanol solution (take preparation of 100mL as an example). Measure 5mL methanol and 95mL water and mix well;
...
