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Forensic science - Methods for detection of rbcL gene of diatom - Capillary electrophoresis and fluorescence method
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Basic data
| Standard ID | GA/T 1965-2021 (GA/T1965-2021) |
| Description (Translated English) | Forensic science - Methods for detection of rbcL gene of diatom - Capillary electrophoresis and fluorescence method |
| Sector / Industry | Public Security (Police) Industry Standard (Recommended) |
| Classification of Chinese Standard | A92 |
| Word Count Estimation | 11,138 |
| Issuing agency(ies) | Ministry of Public Security |
GA/T 1965-2021: Forensic science - Methods for detection of rbcL gene of diatom - Capillary electrophoresis and fluorescence method
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Forensic science - Methods for detection of rbcL gene of diatom - Capillary electrophoresis and fluorescence method
ICS 13.310
CCSA92
People's Republic of China Public Safety Industry Standards
Detection of Specific Fragments of Diatom rbcL Gene in Forensic Science
Capillary Electrophoresis Fluorescence Detection
Published on 2021-10-14
2022-05-01 Implementation
Published by the Ministry of Public Security of the People's Republic of China
foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
drafted.
Please note that some content of this document may be patented. The issuing agency of this document assumes no responsibility for identifying patents.
This document is proposed and managed by the National Standardization Technical Committee of Criminal Technology (SAC/TC179).
This document was drafted by. Guangzhou Institute of Criminal Science and Technology, Sun Yat-sen University, Guangdong Provincial Public Security Department.
The main drafters of this document. Liu Chao, Xu Quyi, Liu Hong, Zhao Jian, Xiao Cheng, Li Yue, Wang Guansan, Liu Changhui, Yang Xingyi, Xu Jichao.
Detection of Specific Fragments of Diatom rbcL Gene in Forensic Science
Capillary Electrophoresis Fluorescence Detection
1 Scope
This document specifies the material preparation, operation method, polymerase chain reaction (Poly-
Light quantitative test results judgment and precautions.
This document applies to the examination of the diatom rbcL gene fragment in forensic examination.
2 Normative references
The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, dated citations
documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to
this document.
GA/T 382-2014 Specification for the construction of forensic science DNA laboratories
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
rbcLgene rbcLgene
Chloroplast ribulose-1,5-bisphosphate carboxylase large subunit gene.
4 Principles
During drowning, damage to the alveolar-capillary barrier causes plankton (such as diatoms) in the water to enter the blood circulation with the drowning fluid, thereby
reach all organs in the human body. Detecting the presence of these plankton can be used to diagnose drowning. drowning related diatoms (Navy, Rhizoma, Ringlet
The chloroplasts of algae, Linear algae, Nephron algae, Osteotheca, etc.) contain the rbcL gene, which has no homology with humans and symbiotic bacteria.
sex. The detection of specific fragments of this gene in the systemic organs (liver or kidney) of cadavers in water can confirm the deoxynucleus of drowning-related diatoms
5 Instruments and equipment
include.
a) Adjustable micropipettes (2μL, 10μL, 100μL, 1000μL);
b) High-speed desktop refrigerated centrifuge, temperature controllable to 4°C and centrifugal speed up to 12000r/min or more;
c) PCR amplifier;
d) Real-time fluorescence quantitative PCR amplification instrument;
e) capillary electrophoresis apparatus;
...
