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GA/T 1964-2021: Forensic science - STR multiplex amplification examination methods for Sus scrofa f. domestica - Capillary electrophoresis and fluorescence method
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GA/T 1964-2021: Forensic science - STR multiplex amplification examination methods for Sus scrofa f. domestica - Capillary electrophoresis and fluorescence method

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Forensic science - STR multiplex amplification examination methods for Sus scrofa f.domestica - Capillary electrophoresis and fluorescence method ICS 13.310 CCSA92 People's Republic of China Public Safety Industry Standards Forensic scientist pig STR complex amplification test Capillary Electrophoresis Fluorescence Detection Published on 2021-10-14 2022-05-01 Implementation Published by the Ministry of Public Security of the People's Republic of China

foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" drafted. Please note that some content of this document may be patented. The issuing agency of this document assumes no responsibility for identifying patents. This document is proposed and managed by the National Standardization Technical Committee of Criminal Technology (SAC/TC179). This document was drafted by. Ministry of Public Security Material Evidence Identification Center, Haikou City Public Security Bureau, Nanjing Forest Police College, Zhanjiang City Public Security Bureau. The main drafters of this document. Zhang Ying, Ma Wenhua, Zhang Jin, Jin Xin, Ma Yanjun, Liu Kaihui, Li Wanshui, Lin Dajun. Forensic scientist pig STR complex amplification test Capillary Electrophoresis Fluorescence Detection

1 Scope

This document specifies the testing principles, instruments, reagents, procedures for individual identification and paternity testing of domestic pigs by compound amplification and fluorescence detection. Precautions in the method of operation, interpretation of results and inspection. This document applies to the STR typing test of domestic pig genome deoxyribonucleic acid (DNA).

2 Normative references

The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, dated citations documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to this document. GA/T 382-2014 Specification for the construction of forensic science DNA laboratories

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 shorttandemrepeats; STR The repeating unit consists of 2 to 6 nucleotides, and the DNA fragment repeats more than 5 to 60 times, and the total length is mostly below 400bp. DNA sequence. 3.2 A technique for amplifying specific DNA fragments in vitro, which can obtain nearly one million copies of target DNA fragments within a few hours. 3.3 Primer The synthetic oligonucleotide sequence is complementary to the sequence flanking the target fragment, which limits the length of the polymerase chain reaction (PCR) product. degree and specificity. 3.4 positive control positivecontrol DNA samples that are known to be typed in the experimental setting are primarily used to monitor the reliability and accuracy of DNA typing results. 3.5 negative control A test sample that does not contain DNA samples set up in an experiment.

4 Principles

Domestic pigs are multiparous and high-yielding mammals belonging to the family Suidae and the genus Porcine. ...