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Forensic science - Methods for SSR detection of species identification of Papaver somniferum L. - Capillary electrophoresis and fluorescence method
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Basic data
| Standard ID | GA/T 1963-2021 (GA/T1963-2021) |
| Description (Translated English) | Forensic science - Methods for SSR detection of species identification of Papaver somniferum L. - Capillary electrophoresis and fluorescence method |
| Sector / Industry | Public Security (Police) Industry Standard (Recommended) |
| Classification of Chinese Standard | A92 |
| Word Count Estimation | 11,127 |
| Issuing agency(ies) | Ministry of Public Security |
GA/T 1963-2021: Forensic science - Methods for SSR detection of species identification of Papaver somniferum L. - Capillary electrophoresis and fluorescence method
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Forensic science - Methods for SSR detection of species identification of Papaver somniferum L.- Capillary electrophoresis and fluorescence method
ICS 13.310
CCSA92
People's Republic of China Public Safety Industry Standards
Forensic Science Poppy Species SSR Marker Detection
Capillary Electrophoresis Fluorescence Detection
Published on 2021-10-14
2022-05-01 Implementation
Published by the Ministry of Public Security of the People's Republic of China
foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
drafted.
Please note that some content of this document may be patented. The issuing agency of this document assumes no responsibility for identifying patents.
This document is proposed and managed by the National Standardization Technical Committee of Criminal Technology (SAC/TC179).
This document is drafted by. the Ministry of Public Security's Physical Evidence Identification Center, the Supreme People's Procuratorate's Procuratorial Technology Information Research Center, and the Bijie City Public Security Bureau.
The main drafters of this document. Xu Xiaoyu, Pei Li, Zhang Ying, Wang Baishi, Zhang Jin, Tu Zheng, Ye Jian, Gao Shan, Liu Kaihui, Peng Jianxiong, Xie Qun,
Go Wendong, Ni Pingya, Song Bingke, Li Yuanyuan, Zhu Shijing.
Forensic Science Poppy Species SSR Marker Detection
Capillary Electrophoresis Fluorescence Detection
1 Scope
This document specifies forensic science deoxyribonucleic acid (DNA) laboratories by polymerase chain reaction (PCR) amplification, capillary electrophoresis
Material preparation, operation method and result judgment for the identification of poppy species by fluorescence detection.
This document applies to the inspection of opium poppy species.
2 Normative references
The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, dated citations
documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to
this document.
GA/T 382-2014 Specification for the construction of forensic science DNA laboratories
GA/T 1160-2014 Silica method for DNA extraction of common drug original plants
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
positive control positivecontrol
Control reactions performed with the addition of known typed template DNA to the PCR system.
3.2
negative control
Control reactions without template DNA but with all other components of the PCR system.
4 Principles
The adult poppy plant is similar to other plants of the same genus in appearance and shape, and it is difficult to distinguish accurately. The morphine content of poppy seedlings is extremely low, and it cannot pass
Chemical composition testing. The SSR loci in this document are opium poppy species-specific and can distinguish opium poppy from other plants, each locus in
Variation in the length of amplified fragments was absent in all poppy individuals.
5 Equipment
Equipment includes.
a) pipettes;
b) benchtop centrifuge;
c) Amplifier;
d) Automatic fluorescence analyzer.
...
