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Method for DNA barcode - Part 2: Quarantine Tetropium spp.
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SN/T 4876.2-2017
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Basic data | Standard ID | SN/T 4876.2-2017 (SN/T4876.2-2017) | | Description (Translated English) | Method for DNA barcode - Part 2: Quarantine Tetropium spp. | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Word Count Estimation | 11,147 | | Date of Issue | 2017-11-07 | | Date of Implementation | 2018-06-01 | | Quoted Standard | SN/T 2013 | | Regulation (derived from) | National Quality Inspection (2017) No. 481 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard stipulates the method for DNA barcode detection and identification of quarantine Broken Eyes. This standard is applicable to the determination of the DNA barcode of the phytosanitary eyebrows in China and the comparison and determination of the results. | SN/T 4876.2-2017: Method for DNA barcode - Part 2: Quarantine Tetropium spp.---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Method for DNA barcode - Part 2. Quarantine Tetropium spp.
People's Republic of China entry and exit inspection and quarantine industry standards
DNA barcode method
Part 2. Quarantine Broken Eyes
Released on.2017-11-07
2018-06-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 4876 "DNA Barcode Method" is divided into five parts.
--- Part 1. Quarantine termites;
--- Part 2. Quarantine Broken Eyes;
--- Part 3. Quarantine moths;
--- Part 4. Quarantine sorghum;
--- Part 5. Mandala;
This part is the second part of SN/T 4876.
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents.
This part is proposed and managed by the National Certification and Accreditation Supervision Committee.
This section drafted by. Jiangsu Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this section. Xu Mei, Qian Lu, Wu Jing, Qi Haixin, Ye Liangling, Ji Qinqin, Zhu Linjuan, Yang Xiaojun, Zheng Sizhu, An Yulin.
DNA barcode method
Part 2. Quarantine Broken Eyes
1 Scope
This part of SN/T 4876 stipulates the method for DNA barcode detection and identification of quarantine Broken Eyed Cattle in China.
This part is applicable to the determination of DNA barcodes of imported phytosanitary Echinococcus chinensis in China, and the comparative analysis and judgment of the results.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
SN/T 2013 Quarantine Identification Method for Dark Brown Eyes
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
DNA barcode DNAbarcode
A short, standardized gene fragment used to distinguish between different species. The fragment has significant genetic variation and differentiation between species.
Easy PCR amplification. DNA barcode technology is mainly used for species identification and new species discovery.
3.2
Voucher specimen voucherspecimen
The physical reference voucher of the DNA barcode, the voucher specimen information includes collection information, collection information, and identification information for review. mode
Specimens are specimens of voucher used for taxonomic research.
3.3
CO I gene
The protein-coding gene of the mitochondrial genome of COIgene, which is called cytochrome c oxidase subunit I (cytochromecoxi-
DasesubunitI), because of its rapid evolution rate, is often used to analyze families of closely related species, subspecies, and geographic populations.
Relationship.
4 Quarantine broken eyed beetles basic information
Chinese name. Broken Eyed Cow
Scientific name. Tetropiumspp.
Taxonomic status. Coleoptera, Chrysomeloidea, Cerambycidae, Tet-Echinococcus
Ropium
Broken eye beetle is an important type of dry pest, most of which are seriously damaging softwood and occasionally can damage broadleaf
wood. It is mainly distributed in Eurasia and the Americas, with 7 species in the Gubei District and 6 species in North America. The main types of quarantine broken eyed beetles in China
There are T. fuscum, T. cinnamopterum, L. gabrieli, and Shih
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