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SN/T 4859-2017: Identification of Mycobacterium tuberculosis complex at frontier port
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Identification of Mycobacterium tuberculosis complex at frontier port
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SN/T 4859-2017
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Basic data | Standard ID | SN/T 4859-2017 (SN/T4859-2017) | | Description (Translated English) | Identification of Mycobacterium tuberculosis complex at frontier port | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Word Count Estimation | 10,151 | | Date of Issue | 2017-07-21 | | Date of Implementation | 2018-03-01 | | Quoted Standard | GB 19489 | | Regulation (derived from) | State-Quality-Inspection-Accreditation (2017) 337 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the biosafety requirements, identification procedures, identification methods, and results reports for the identification of M. tuberculosis complexes at border ports. This standard is applicable to the detection and identification of the infection of Mycobacterium tuberculosis complexes at the border ports. | SN/T 4859-2017: Identification of Mycobacterium tuberculosis complex at frontier port---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of Mycobacterium tuberculosis complex at frontier port
People's Republic of China entry and exit inspection and quarantine industry standards
Identification method of Mycobacterium tuberculosis complex group at border port
Released on.2017-07-21
2018-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Lu Yi, Wang Jian, Zhang Qi, Fang Wei, Meng Chengyan, Ye Wei, Cha, Jia Zhe, Zhang Xiaohang, Han Xiaohui, Zhou Wei,
Qiu Wei.
Introduction
In recent years, new technologies for tuberculosis laboratory diagnosis have emerged, achieving rapid and accurate detection of Mycobacterium tuberculosis and its drug resistance.
Standard. At present, China is conducting tuberculosis diagnosis based on the "WS288-2008 diagnostic criteria for tuberculosis", in which the laboratory diagnosis of tuberculosis remains the main
It relies on sputum smear microscopy and traditional solid culture. Microscopy is less sensitive and cannot distinguish between active and inactive
The organism cannot identify the type of mycobacteria. On traditional solid media, the growth of Mycobacterium tuberculosis takes up to eight weeks, reporting time
long. In the absence of timely diagnosis, appropriate treatment is delayed. At the same time, laboratories need fast and inexpensive methods of culture identification.
It is used to distinguish between Mycobacterium tuberculosis complex and non-tuberculous mycobacteria. WHO's standards for the diagnosis of tuberculosis continue to improve,
The proper integration of new technologies and methods into diagnostic protocols is one of the prerequisites for the effective adoption of new diagnostic methods.
Based on the full evaluation, this standard regulates the application path of M. tuberculosis laboratory identification technology and comprehensive judgment scheme.
Through the adoption of new technologies, the prevention and control efficiency of tuberculosis at border ports has been improved.
Identification method of Mycobacterium tuberculosis complex group at border port
1 Scope
This standard specifies the biosafety requirements, identification procedures, identification methods, and results reports for the identification of Mycobacterium tuberculosis complexes at border ports.
And so on.
This standard is applicable to the detection and identification of the infection of Mycobacterium tuberculosis complexes at the border ports.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
Tuberculosis Laboratory Biosafety Handbook World Health Organization
List of pathogenic microorganisms transmitted by humans
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Mycobacterium tuberculosis complex; MTC
A group of pathogenic bacteria of infectious tuberculosis, including Mycobacterium tuberculosis, Mycobacterium tuberculosis, Mycobacterium vaccae, African branching rods
bacteria. The bacteria of MTC are rod-shaped, the size of the bacteria is 0.6μm × (1μm ~ 4μm), positive for acid-fast staining and positive for Gram stain. Slow growth
Slow, about 10h breeding generation, according to the amount of inoculation, colony growth can be seen in the naked eye for 10d~30d. Lowenstein-Jensen culture
The colonies on the base are rough and dense, and are granules, nodules, or cauliflower-like, milky or beige, and opaque.
4 Abbreviations
The following abbreviations apply to this document.
PAGE (polyacrylamide gel electrophoresis). polyacrylamide gel electrophoresis
TBE (Tris-Boricacid). Tris-boric acid nucleic acid running buffer
Bp(basepair). base pair
5 Biosecurity measures
According to the requirements of the WHO Tuberculosis Laboratory Biosafety Manual and GB 19489 and the List of Pathogenic Microorganisms Infected by Humans,
The biosafety requirements for the identification and identification of materials for mycobacterial complexes are as follows.
--- Sample processing and testing should be carried out in Biosafety Level 2 (BSL-2) laboratories, in Class IIA or above biosafety cabinets;
--- A large number of live bacteria operations, such as culture identification, drug sensitivity testing, etc., should be in the biosafety level 3 (BSL-3) laboratory or tuberculosis protection
Performed in a biological safety cabinet in the laboratory. The tuberculosis protection laboratory refers to the experiment based on the biosafety secondary laboratory.
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