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Identification of the ingredients of common small berry fruits in food and juice for export. Real-time PCR method - Part 4: Mulberry
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SN/T 4849.4-2017
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Basic data Standard ID | SN/T 4849.4-2017 (SN/T4849.4-2017) | Description (Translated English) | Identification of the ingredients of common small berry fruits in food and juice for export. Real-time PCR method - Part 4: Mulberry | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | X50 | Word Count Estimation | 8,833 | Date of Issue | 2017-07-21 | Date of Implementation | 2018-03-01 | Regulation (derived from) | State-Quality-Inspection-Accreditation (2017) 337 | Issuing agency(ies) | General Administration of Customs |
SN/T 4849.4-2017: Identification of the ingredients of common small berry fruits in food and juice for export. Real-time PCR method - Part 4: Mulberry ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Method for the detection of common small berries components in food and beverages - Real - time fluorescent PCR method - Part 4. Mulberry)
People's Republic of China entry and exit inspection and quarantine industry standards
Common small berries in foods and beverages
Detection method real-time fluorescent PCR
Part 4. Mulberry
Part 4. Mulberry
Released on.2017-07-21
2018-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 4849 "Detection method for common small berries in export foods and beverages, real-time fluorescent PCR method" series of standards
Part 6.
--- Part 1. Blueberry;
--- Part 2. Raspberry;
--- Part 3. Blackcurrant;
--- Part 4. Mulberry;
--- Part 5. Cranberry and Blueberry;
--- Part 6. Kiwifruit.
This part is the fourth part of SN/T 4849.
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents.
This part is proposed and managed by the National Certification and Accreditation Administration of the People's Republic of China.
This section drafted by. China Academy of Inspection and Quarantine, the People's Republic of China Altay Entry and Exit Inspection and Quarantine Bureau.
The main drafters of this section. Huang Wensheng, Chen Ying, Deng Tingting, Wu Yajun, Yang Yange, Li Fei, Zhang Jiukai, Han Jianxun, Wang Bin.
Common small berries in foods and beverages
Detection method real-time fluorescent PCR
Part 4. Mulberry
1 Scope
This part of SN/T 4849 specifies the real-time fluorescent PCR method for the detection of mulberry ingredients in foods and beverages.
This section applies to fresh fruit, frozen fruit, candied fruit, freshly squeezed juice, pulp-type juice, dried fruit, jam and other raw foods with mulberry as raw materials.
Qualitative testing of mulberry ingredients in products and beverages (except clear juice and fruit wine). The minimum detection limit (LOD) for the method specified in this section is 1%
(Volume ratio).
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 27403-2008 Laboratory quality control regulations Food molecular biology testing
3 terms and definitions, abbreviations
3.1 Terms and definitions
The following terms and definitions apply to this document.
3.1.1
Small berry smallberryfruits
Berry refers to a soft, juicy fleshy single fruit that is developed from an ovary or in combination with other flower organs and is formed by one or several carpels. small
Berry refers to a small, juicy type of berry.
3.1.2
Mulberry mulberry
The fruit of the genus Moraceae, which is a conglomerate, which is composed of most of the oval-shaped small drupes. It is oblong, yellow-brown, brown-red to
Dark purple, (some varieties are milky white when mature). Latin name. MorusalbaL.
3.2 Abbreviations
The following abbreviations apply to this document.
DNA. deoxyribonuleic acid
PCR. polymerase chain reaction (polymerase chain reaction)
Ct value. number of cycles experienced when the fluorescent signal in each reaction tube reaches the set domain value
dNTP. deoxyribonuleosidetriphosphate
dATP. deoxyadenosine triphosphate (deoxyadenosinetriphosphate)
dCTP. deoxycytidine triphosphate (deoxycytidinetriphosphate)
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