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SN/T 4827-2017: Quarantine protocol for Chryseobacterium meningosepticum of frog
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 4827-2017 | English | 279 |
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Quarantine protocol for Chryseobacterium meningosepticum of frog
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SN/T 4827-2017
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Basic data | Standard ID | SN/T 4827-2017 (SN/T4827-2017) | | Description (Translated English) | Quarantine protocol for Chryseobacterium meningosepticum of frog | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Word Count Estimation | 12,188 | | Date of Issue | 2017-07-21 | | Date of Implementation | 2018-03-01 | | Regulation (derived from) | State-Quality-Inspection-Accreditation (2017) 337 | | Issuing agency(ies) | General Administration of Customs | SN/T 4827-2017: Quarantine protocol for Chryseobacterium meningosepticum of frog---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Technical specification for quinatal brucellosis in frog meningitis)
People's Republic of China entry and exit inspection and quarantine industry standards
Technical specification for quarantine of frog meningitis
Released on.2017-07-21
2018-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Xiamen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Jiangsu Province Entry and Exit Inspection
Epidemic situation.
The main drafters of this standard. Xu Shufei, Kong Fande, Xie Mingxing, Tang Taishan, Wang Kaimin, Jiang Yan.
Technical specification for quarantine of frog meningitis
1 Scope
This standard specifies the clinical symptoms and bacteriology, PCR and other laboratory diagnosis and detection methods for frog meningitis.
This standard is applicable to the epidemiological investigation, monitoring, diagnosis and entry-exit inspection and quarantine of frog meningitis.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 18088 entry and exit animal quarantine sampling
3 reagents and media
Unless otherwise specified, the following biochemical reagents were of analytical grade. See Appendix A for the reagent preparation method in the text.
3.1 Nutritional broth.
3.2 Tryptone soy agar (TSA).
3.3 thiosulfate citrate bile salt sucrose agar (TCBS).
3.4 Nutritional agar.
3.5 Biochemical identification kit.
3.6 Gram staining solution.
3.7 DNA extraction reagents. related reagents required for proteinase K, SDS, phenol-chloroform extraction; commercial DNA extraction kit.
3.8 PCR reagent. 10× concentrated buffer, dNTP, magnesium chloride, 6× electrophoresis loading buffer, agarose, 5× running buffer (TBE)
Etc., all of the ingredients in the commercial kit.
3.9 Primers.
a) Specific primers.
---P1.5'-GATTCGTCGGATTATATTG-3';
---P2. 5'-CCACTTCAACCTTACTCAAGACTAAC-3'.
The concentration was 10 μmol/L, and the size of the amplified product was 475 bp to 479 bp.
b) Universal primers.
---P3. 5'-AGAGTTTGATCMTGGCTCAG-3';
---P4. 5'-GGYTACCTTGTTACGACTT-3'.
The concentration was 10 μmol/L, and the size of the amplified product was 1488 bp.
3.10 Negative and positive controls. provided by the designated laboratory of the AQSIQ.
3.11 DNADL2000Marker. It is suitable for detecting DNA fragments between 100bp and.2000bp in molecular weight and stored at -20°C.
4 Disease Overview
Frog meningitis, septicemia, caused by meningitis, Elizabethkingiameningoseptica
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