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Test methods for Plasmodium by real time PCR at ports
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SN/T 4793-2017
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Basic data Standard ID | SN/T 4793-2017 (SN/T4793-2017) | Description (Translated English) | Test methods for Plasmodium by real time PCR at ports | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | C62 | Word Count Estimation | 10,169 | Date of Issue | 2017-05-12 | Date of Implementation | 2017-12-01 | Regulation (derived from) | State-Quality-Inspection-Accreditation (2017) 228 | Issuing agency(ies) | General Administration of Customs |
SN/T 4793-2017: Test methods for Plasmodium by real time PCR at ports---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Detection of Plasmodium falciparum by Real - time Fluorescence)
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Real-time fluorescent PCR detection method for malaria parasites at border ports
Released on.2017-05-12
2017-12-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shantou Entry-Exit Inspection and Quarantine
Bureau, Guangzhou Airport Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Shi Yongxia, Li Xiaobo, Zhu Junxian, Li Yan, Huang Jicheng, Xing Luqin, Hong Wei, Sun Wei, Zhong Yuqing, Xiang Dapeng.
Real-time fluorescent PCR detection method for malaria parasites at border ports
1 Scope
This standard specifies the biosafety requirements for malaria detection at border ports, the collection, transportation and preservation of specimens, and real-time fluorescent PCR for malaria.
Test method.
This standard is applicable to real-time fluorescent PCR of suspected falciparum malaria, vivax malaria, ovarian malaria and three-day malaria infected persons at border ports.
Detection.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
List of pathogenic microorganisms transmitted from humans (Weike Jiaofa [2006] No. 15)
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Malaria malaria
Parasitic diseases caused by human Plasmodium infection are mainly transmitted by female Anopheles bites. Plasmodium first invades liver cells to develop and reproduce, and then
Invasion of red blood cells to breed, causing red blood cells to burst and disease. Clinically, after repeated episodes of intermittent chills, high fever, followed by sweating
Mitigation is characteristic.
3.2
Plasmodium Plasmodium
It belongs to Eucoccidida, Plasmodidae, Plasmodium, and is the causative agent of malaria.
body. There are four species of parasitic parasites in humans, namely Plasmodiumvivax and Plasmodiumfalci-
Parum), Plasmodium malariae and Plasmodiumovale, causing vivax malaria
Sexual malaria, three-day malaria and oval malaria.
4 Abbreviations
The following abbreviations apply to this document.
Real-time fluorescent RT-PCR. real-time fluorescent reverse transcription-polymerase chain reaction.
Ct value. The number of cycles experienced when the fluorescent signal in each reaction tube reaches a set threshold.
DNA. Deoxyribonucleic acid.
FAM. FAM fluorescent dye, a fluorescent reporter group.
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