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SN/T 4734-2016: Detection and identification of Phytophthora lateralis Tucker et Mibrath
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Detection and identification of Phytophthora lateralis Tucker et Mibrath
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SN/T 4734-2016
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Basic data | Standard ID | SN/T 4734-2016 (SN/T4734-2016) | | Description (Translated English) | Detection and identification of Phytophthora lateralis Tucker et Mibrath | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 14,159 | | Date of Issue | 2016-12-12 | | Date of Implementation | 2017-07-01 | | Regulation (derived from) | State-Quality-Inspection-Accredidation (2016) No.590 | | Issuing agency(ies) | General Administration of Customs | SN/T 4734-2016: Detection and identification of Phytophthora lateralis Tucker et Mibrath---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Phytophthora lateralis Tucker et Mibrath
China's entry-exit inspection and quarantine industry standards
Phytosanitary identification method for phytophthora stipules root rot
Published on.2016-12-12
2017-07-01 Implementation
China
The State Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. Publication of this document
The structure does not assume responsibility for identifying these patents.
This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission.
Drafters of this standard. Kunshan Entry-Exit Inspection and Quarantine Bureau, Nanjing Agricultural University, People's Republic of China, Jiangsu Province
Entry Inspection and Quarantine Bureau.
The main drafters of this standard. Ji Rui, Zhang Zhengguang, Liao Tailin, Li Baisheng, Wang Jiansheng, An Yulin, Wu Cuiping.
Phytosanitary identification method for phytophthora stipules root rot
1 Scope
This standard specifies Phytophthora lateralis Tucker
This standard applies to entry and exit seedlings, logs, timber, wood packaging and other plant materials and their soil and media in Phytophthora capsici
Quarantine and identification.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
SN/T 2122 Quarantine sampling of entry and exit plants and plant products
3 Phytophthora capsici root rot basic information
Scientific name. Phytophthora lateralis Tucker
Taxonomic status. Chromista, Oomycota, Peronosporales, Pythiaceae, Phytophthora
Phytophthora.
See Appendix A for additional information on Phytophthora capsici.
4 Methodology
Based on the isolation and culture of pathogens, morphological analysis of the isolated pathogens and nested PCR detection were performed.
Strip size to determine the result.
5 Equipment and main reagents
5.1 Instruments and Equipment
PCR apparatus, ultra-clean bench, sterilizer, ice maker, high-speed refrigerated centrifuge, bench-top small centrifuge, conventional refrigerator, vortex oscillator,
Microsampler, electrophoresis, gel imaging system.
5.2 Main Reagents
Unless otherwise specified, all reagents are analytical grade and biochemical grade reagents. The experimental water is ultrapure water, and the specifications are in accordance with GB/T 6682 phase.
Related regulations.
V8 juice, calcium carbonate, agar powder, penicillin, rifamycin, pentachloronitrobenzene, cetyltrimethylammonium bromide (CTAB), lauryl sulfur
Sodium (SDS), anhydrous ethanol, phenol, chloroform, isoamyl alcohol, sodium acetate, RNase, sodium hydroxide, Tris, PCR
Buffer, MgCl2, dNTPs (containing dATP, dTTP, dCTP, dGTP), Taq DNA polymerase, primers T1/T2, primer T3/T4,
DL2000 marker, running buffer, agarose, ethidium bromide (EB).
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