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SN/T 4708-2016: Detection method of vibrio cholerae and vibrio mimicus by duplex real-time PCR at frontier ports
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| SN/T 4708-2016 | English | 229 |
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Detection method of vibrio cholerae and vibrio mimicus by duplex real-time PCR at frontier ports
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SN/T 4708-2016
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Basic data | Standard ID | SN/T 4708-2016 (SN/T4708-2016) | | Description (Translated English) | Detection method of vibrio cholerae and vibrio mimicus by duplex real-time PCR at frontier ports | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Word Count Estimation | 10,174 | | Date of Issue | 2016-12-12 | | Date of Implementation | 2017-07-01 | | Regulation (derived from) | State-Quality-Inspection-Accredidation (2016) No.590 | | Issuing agency(ies) | General Administration of Customs | SN/T 4708-2016: Detection method of vibrio cholerae and vibrio mimicus by duplex real-time PCR at frontier ports
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Detection method of vibrio cholerae and vibrio mimicus by duplex real-time PCR at frontier ports
People's Republic of China Exit Inspection and Quarantine standards
Vibrio cholerae and Vibrio mimicus double fluorescence at the border crossing
PCR detection method
2016-12-12 released
2017-07-01 implementation
People's Republic of China
General Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard drafting unit. China Inspection and Quarantine Science Institute, People's Republic of China Jiangsu Exit Inspection and Quarantine.
The main drafters of this standard. Han Hui, Yang Yu, Wu Hailei, Tan Ke, Song Yan Beijing, Li Shan, Xu Baoliang.
Vibrio cholerae and Vibrio mimicus double fluorescence at the border crossing
PCR detection method
1 Scope
This standard specifies the border crossing Vibrio cholerae and mimic Vibrio testing methods, including the detection of objects, sample collection and processing, the operator
Law and Results Report.
This standard applies to entry and exit personnel at border crossings, environmental water carrying Vibrio cholerae (including O1 group, O139 group and non-O1 group /
O139 Vibrio cholerae) and mimicry Vibrio molecular biology tests.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
For all undated references, the latest edition (including all amendments) applies to this document.
GB/T 19489-2008 Laboratory biosafety general requirements
General Principles of Biosafety in Microbiology and Biomedical Laboratories
Regulations on the Transport and Administration of Highly Pathogenic Microbial (Poisonous) Bacteria or Species That Can Infect Human Beings (Ministry of Health Decree No. 45)
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Vibrio Cholerae
Vibrio, Gram stain negative. Patients and carriers are the main source of cholera, contaminated by excrement or excrement from patients and carriers
Water or food can cause cholera outbreak. Cholera has the heat-resistant O antigen and the heat-labile H antigen. According to different O antigen, Huo
Vibrio cholerae can be divided into more than.200 serotypes, of which only O1 and O139 Vibrio cholerae strains can cause cholera epidemic.
3.2
Vibrio mimicus
Vibrio, Gram stain negative. Widely found in nature water, sea water and seafood. Is a human and aquatic animal disease
Pathogens, which infect humans through water and aquatic products, have become pathogenic to sporadic and epidemic diarrhea and food poisoning in some areas
one. Vibrio mimicry biochemical characteristics and Vibrio cholerae are very similar, mainly caused by human acute diarrhea.
4 Abbreviations
The following abbreviations apply to this document.
PCR. Polymerase chain reaction
DNA. DNA
DEPC. Dicobal
FAM.6-carboxy-fluorescein, a fluorescent reporter group
HEX. Hexachloro-6-methylfluorescein, a fluorescent reporter
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