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SN/T 4656.6-2017: Biosafety testing methods of the textiles for import and export - Part 6: Salmonella
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| SN/T 4656.6-2017 | English | 419 |
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Biosafety testing methods of the textiles for import and export - Part 6: Salmonella
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SN/T 4656.6-2017
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Basic data | Standard ID | SN/T 4656.6-2017 (SN/T4656.6-2017) | | Description (Translated English) | Biosafety testing methods of the textiles for import and export - Part 6: Salmonella | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | W04 | | Classification of International Standard | 59.080 | | Word Count Estimation | 18,175 | | Date of Issue | 2017-11-07 | | Date of Implementation | 2018-06-01 | | Quoted Standard | GB 19489; GB/T 27403; SN/T 1538.1; SN/T 1538.2 | | Regulation (derived from) | National-Quality-Inspection-Accrediatation (2017) 481 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the inspection method for Salmonella in imported and exported textiles. This standard applies to the inspection of imported and exported textile Salmonella. | SN/T 4656.6-2017: Biosafety testing methods of the textiles for import and export - Part 6: Salmonella
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Biosafety testing methods of the textiles for import and export - Part 6. Salmonella
People's Republic of China entry and exit inspection and quarantine industry standards
Import and export textile biosafety inspection method
Part 6. Salmonella
Part 6. Salmonella
Released on.2017-11-07
2018-06-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Entry-Exit Inspection and Quarantine
Industry Standard
Import and export textile biosafety inspection method
Part 6. Salmonella
China Standard Press Publishing
First edition, July.2018
Foreword
SN/T 4656 "Inspection of Import and Export Textile Biosafety" is divided into six parts.
--- Part 1. Candida albicans;
--- Part 2. Escherichia coli;
--- Part 3. Coliforms;
--- Part 4. Staphylococcus aureus;
--- Part 5. Total number of colonies;
--- Part 6. Salmonella.
This part is the sixth part of SN/T 4656.
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. Henan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Hebei Province Entry and Exit Inspection
Epidemic situation.
The main drafters of this section. Li Wei, Guo Huiqing, Yan Jianying, Xu Chao, Wang Lei, Zhang Qin, Miao Li, Qiao Qing, Sun Xiaoxia.
Import and export textile biosafety inspection method
Part 6. Salmonella
1 Scope
This part of SN/T 4656 specifies the method for the detection of Salmonella in imported and exported textiles.
This section applies to the inspection of imported and exported textile Salmonella.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
GB/T 27403 Laboratory Quality Control Specification for Food Molecular Biology Testing
SN/T 1538.1 Guidelines for the preparation of medium - Part 1
SN/T 1538.2 Medium Preparation Guide Part 2. A Practical Guide to Medium Performance Testing
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Textile biosafety biosafetyoftextiles
Potential threats to the ecological environment and human health caused by the harmful organisms carried by the textiles themselves or their metabolites
Take a series of effective prevention and control measures.
3.2
Salmonella Salmonella
A pathogen of zoonotic disease is a Gram-negative Enterobacter. This mushroom has low nutritional requirements and is widely distributed in nature.
It can be detected in food, water, soil, animal hair, fiber, and can infect humans and many kinds of animals, most of which are highly pathogenic. Currently issued
There are nearly 3,000 serotypes of Salmonella, many of which can cross-infect between humans and animals, causing human and animal
A variety of different clinical manifestations, mainly caused by fever, gastroenteritis, diarrhea and sepsis, have caused serious harm to human health.
3.3
Loop-mediated constant temperature amplification loop-mediated isothermalamplification; LAMP
Two pairs of specific internal and external primers designed according to the target gene-specific target sequence conserved gene, specifically identifying six independent targets on the target sequence
In the region, Bst DNA polymerase was used to initiate the cyclic chain displacement reaction, and the isothermal condition (about 63 ° C) was kept for 30 min to 60 min.
Different, efficient, and rapid completion of nucleic acid amplification. Pyrophosphate ions precipitated from dNTP combine with Mg2 in the reaction solution to produce by-products
The substance (magnesium pyrophosphate) forms a milky white precipitate, and the coloring solution is added to observe the judgment result by color change.
4 equipment, materials
4.1 Basic equipment and materials for routine microbiological testing.
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