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Determination of mycotoxins in Chinese herbal medicine for import and export
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SN/T 4604-2016
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PDF similar to SN/T 4604-2016
Standard similar to SN/T 4604-2016 GB/T 41907 SN/T 4806 SN/T 4527 SN/T 4458
Basic data Standard ID | SN/T 4604-2016 (SN/T4604-2016) | Description (Translated English) | Determination of mycotoxins in Chinese herbal medicine for import and export | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | C23 | Word Count Estimation | 18,140 | Date of Issue | 2016-08-23 | Date of Implementation | 2017-03-01 | Regulation (derived from) | State-Quality-Inspection-Certification (2016)438 | Issuing agency(ies) | General Administration of Customs |
SN/T 4604-2016: Determination of mycotoxins in Chinese herbal medicine for import and export---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Determination of Mycotoxins in Imported and Exported Chinese Medicinal Materials)
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Determination of mycotoxins in Chinese herbal medicines for import and export
Published on.2016-08-23
2017-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Chongqing Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shanghai Entry and Exit Inspection of the People's Republic of China
Epidemic situation.
The main drafters of this standard. Nie Fuping, Tang Baibin, Yang Jun, Qu Cunxian, Wang Guomin, Li Xianliang, Wang Wei, Wu Rui, Tian Jun, Kong Deying,
Yang Huiqin.
Determination of mycotoxins in Chinese herbal medicines for import and export
1 Scope
This standard specifies aflatoxin B1, B2, G1, G2, M1, M2, ochratoxin A, fumonisin B1, B2, T-2 in Chinese herbal medicines for import and export.
Liquid chromatography-mass spectrometry/mass spectrometry for detection of toxins, HT-2 toxins, dihydroxybenzoic acid lactam mycotoxins.
This standard applies to import and export of Angelica, Campanulaceae, Schisandra, Poria, Daqingye, Honeysuckle, Dilong Aflatoxin B1, B2, G1, G2
Liquid color of M1, M2, ochratoxin A, fumonisin B1, B2, T-2 toxin, HT-2 toxin, dihydroxybenzoic acid lactone mycotoxins
Spectral-mass spectrometry/mass spectrometry.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Method summary
The sample is extracted with PBS solution and methanol, and the extract is diluted, filtered, and purified by an immunoaffinity column. After the eluent is blown dry to a constant volume, the extract is used.
Liquid chromatography-mass spectrometry/mass spectrometry, external standard method for quantification.
4 reagent materials
Unless otherwise stated, the reagents used were of analytical grade and the water was the first grade water specified in GB/T 6682.
4.1 Methanol. Chromatographically pure.
4.2 Acetonitrile. chromatographically pure.
4.3 Formic acid. chromatographically pure.
4.4 Ammonium acetate. chromatographically pure.
4.5 Disodium hydrogen phosphate dodecahydrate.
4.6 Potassium chloride.
4.7 Potassium dihydrogen phosphate.
4.8 Sodium chloride.
4.9 Concentrated hydrochloric acid.
4.10 Tween.
4.11 Sodium hydroxide.
4.12 0.1 mol/L hydrochloric acid solution. Measure 9 mL of concentrated hydrochloric acid (4.9) and dilute to 1000 mL with water.
4.13 1 mol/L sodium hydroxide solution. 40.0 g of solid sodium hydroxide (4.11) was weighed, dissolved in water and diluted to 1000 mL.
4.14 pH7.4 PBS solution. Weigh 2.92g of disodium hydrogen phosphate dodecahydrate (4.5), 0.20g of potassium chloride (4.6), 0.20g of potassium dihydrogen phosphate
(4.7) and 8.00g NaCl (4.8) dissolved in 900mL of water, using 0.1mol/L hydrochloric acid solution (4.12) or 1mol/L sodium hydroxide solution
(4.13) Adjust the pH to 7.4 and dilute to 1000 mL with water.
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