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SN/T 4545.1-2016: Commercial kit method - Salmonella - Test method I
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Commercial kit method - Salmonella - Test method I
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SN/T 4545.1-2016
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Basic data | Standard ID | SN/T 4545.1-2016 (SN/T4545.1-2016) | | Description (Translated English) | Commercial kit method - Salmonella - Test method I | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | G66 | | Word Count Estimation | 10,13 | | Date of Issue | 2016-08-23 | | Date of Implementation | 2017-03-01 | | Regulation (derived from) | State-Quality-Inspection-Certification (2016)438 | | Issuing agency(ies) | General Administration of Customs | SN/T 4545.1-2016: Commercial kit method - Salmonella - Test method I---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Commercial kit method-Salmonella-Test method Ⅰ
People's Republic of China Exit Inspection and Quarantine standards
Commercial kit detection method
Salmonella method one
2016-08-23 released
2017-03-01 Implementation
People's Republic of China
General Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This section is part 1 of SN/T 4545.
This section drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of this document may be patentable. The release of this document
The agency does not assume responsibility for identifying these patents.
This part of the national certification and accreditation supervision and management committee proposed and centralized.
This section is drafted by Fujian Entry-Exit Inspection and Quarantine of People's Republic of China, Guangzhou Huafeng Biological Technology Co., Ltd.
The main drafters of this section. Shao Biying, Zheng Jing, Dong Jian, Chen Bin, Huang Xiaorong, Peng Hua Yi, Lin Jie.
Commercial kit detection method
Salmonella method one
1 Scope
This part of SN/T 4545 specifies the detection of Salmonella ring-mediated constant temperature nucleic acid amplification (LAMP) in food.
This section applies to poultry, fish and seafood, dairy products and sundries screening screening test.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB 4789.4 National Food Safety Standard Microbiological Tests for Salmonella
GB 19489 laboratory biosafety common requirements
GB/T 27403 laboratory quality control specification food molecular biology test
SN/T 2775 commercial food testing kit evaluation method
SN/T 3266 Microbiological test methods for the validation of technical specifications
3 Biosecurity measures
To protect the safety of laboratory personnel and prevent contamination, Salmonella should be tested by qualified staff. All cultures,
DNA extracts and waste should be in accordance with GB 19489 and GB/T 27403 in the implementation of the relevant provisions.
4 Technical Overview
Salmonella Screening by Loop-mediated Isothermal Amplification (LAMP)
Detection. Based on 6 independent regions on the Salmonella-specific target sequence, 4 specific primers and a strand-displacement-competent DNA
Polymerase at 65 ℃ to complete the constant temperature amplification. In a ring-mediated isothermal amplification reaction, macroscopic magnesium pyrophosphate was produced as a by-product
Color precipitation, the reaction products in the role of fluorescent dyes, the positive and negative results have a color difference, you can observe the results of the determination of color change.
5 Reagents and materials
5.1 Salmonella nucleic acid LAMP detection kit
The kit reference SN/T 2775 and SN/T 3266 by the CNCA commodity food testing kit Evaluation Committee of Experts Group
Weaving evaluation. Specific evaluation results see Appendix A. The kit is composed as follows.
a) DNA extract.
b) Salmonella HF reaction tube, which is a loop-mediated constant temperature amplification reaction tube, each tube contains 22.5μL working solution (including reaction solution and Bst
DNA polymerase), 2 μL of color developing solution and 50 μL of stabilizing solution.
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