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SN/T 4544.1-2016: Commercial kit method - Aerobic count - Test method I
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 4544.1-2016 | English | 229 |
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Commercial kit method - Aerobic count - Test method I
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SN/T 4544.1-2016
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Basic data | Standard ID | SN/T 4544.1-2016 (SN/T4544.1-2016) | | Description (Translated English) | Commercial kit method - Aerobic count - Test method I | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | G66 | | Word Count Estimation | 10,151 | | Date of Issue | 2016-08-23 | | Date of Implementation | 2017-03-01 | | Regulation (derived from) | State-Quality-Inspection-Certification (2016)438 | | Issuing agency(ies) | General Administration of Customs | SN/T 4544.1-2016: Commercial kit method - Aerobic count - Test method I---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Commercial kit method-Aerobic count-Test method Ⅰ
People's Republic of China Exit Inspection and Quarantine standards
Commercial kit detection method
The total number of colonies method one
2016-08-23 released
2017-03-01 Implementation
People's Republic of China
General Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This part is the first part of SN/T 4544.
This section drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of this document may be patentable. The release of this document
The agency does not assume responsibility for identifying these patents.
This part of the national certification and accreditation supervision and management committee proposed and centralized.
This part of the drafting unit. People's Republic of China Fujian Entry-Exit Inspection and Quarantine, Beijing Landbridge Technology Co., Ltd., the Chinese people
Shaoxing Exit Inspection and Quarantine of the Republic.
The main drafters of this section. Lin Jie, Zheng Jing, Huang Xiaorong, Chen Bin, Dong Jian, Ko Lu, Dai Xiaoli, Ren Mingxing.
Commercial kit detection method
The total number of colonies method one
1 Scope
This section specifies the method of detection of the total number of colonies in food testing (EasyTestTMAerobicCount).
This section applies to meat, fish and seafood, fruits and vegetables, dairy products and sundry foods such as the determination of the total number of colonies.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB 4789.2 National Food Safety Standard Microbiological examination of food - Determination of the total number of colonies
GB 19489 laboratory biosafety common requirements
GB/T 27403 laboratory quality control specification food molecular biology test
Evaluation of Commercial Food Testing Kits
Microbiological method for the validation of foodstuffs - Technical specifications
3 Biosecurity measures
To protect the safety of laboratory personnel and prevent contamination, the total number of colonies should be checked by qualified staff. All cultures and
Waste should be in accordance with GB 19489 and GB/T 27403 in the implementation of the relevant provisions.
4 Technical Overview
The total colony count (EasyTest TMAerobicCount) is a pre-prepared media system containing standard culture
And TTC indicator, colonies red or pink on the test piece, which enhances colony counting.
5 Reagents and materials
5.1 The total number of colonies test piece (EasyTestTMAerobicCount)
This part of the kit used by Beijing Landbridge Technology Co., Ltd. production, refer to SN/T 2775-2011 and SN/T 3266-
2012 by the national commission commissioned commercial food testing kit evaluation committee of experts evaluation. Specific evaluation results see Appendix A.
Kit stored at 2 ℃ ~ 8 ℃, valid for 6 months. The test piece in the kit should be warmed to room temperature before use.
5.2 Phosphate buffer (PBS)
Weigh 34.0g of potassium dihydrogen phosphate was dissolved in 500mL of distilled water, with about 175mL of 1mol/LNaOH solution
Adjust pH, diluted to 1000mL with distilled water and stored in the refrigerator.
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