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SN/T 4542.1-2016: Commercial kit method--Streptomycin and dihydrostreptomycin--Test method Ⅰ
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 4542.1-2016 | English | 229 |
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Commercial kit method--Streptomycin and dihydrostreptomycin--Test method Ⅰ
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SN/T 4542.1-2016
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Basic data | Standard ID | SN/T 4542.1-2016 (SN/T4542.1-2016) | | Description (Translated English) | Commercial kit method--Streptomycin and dihydrostreptomycin--Test method �� | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Word Count Estimation | 10,159 | | Date of Issue | 2016-08-23 | | Date of Implementation | 2017-03-01 | | Regulation (derived from) | State-Quality-Inspection-Certification (2016)438 | | Issuing agency(ies) | General Administration of Customs | SN/T 4542.1-2016: Commercial kit method--Streptomycin and dihydrostreptomycin--Test method Ⅰ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Commercial kit method--Streptomycin and dihydrostreptomycin--Test method I
China's entry-exit inspection and quarantine industry standards
Replacing SN/T 2059-2008
Commercial kit detection method
Streptomycin and Dihydro Streptomycin
method one
Published on.2016-08-23
2017-03-01 Implementation
China
The State Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This section is Part 1 of SN/T 4542.
This section was drafted in accordance with the rules given in GB/T 1.1-2009.
This section replaces SN/T 2059-2008 "Determination of streptomycin and dihydrostreptomycin residues in royal jelly from import and export.
Epidemic law.
The main technical changes in this section and SN/T 2059-2008 are as follows.
--- Standard name changed to "commercial kit detection method streptomycin and dihydrostreptomycin method one."
--- Added appraisal results of the kit in the appendix.
Please note that some of the contents of this document may involve patents. The issuing agency of this document does not assume responsibility for identifying these patents.
This part is proposed and managed by the National Certification and Accreditation Regulatory Commission.
This section was drafted. Zhejiang Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Zhejiang Dean Biotechnology Co., Ltd.
The main drafters of this section. Zhang Xiaofeng, Shao Honghong, Li Ke, Wu Hao, Shuai Jiang Bing, Wang Zizi.
Commercial kit detection method
Streptomycin and Dihydro Streptomycin
method one
1 Scope
This part of SN/T 4542 specifies enzyme-linked immunosorbent assays for streptomycin and dihydrostreptomycin residues in freeze-dried royal jelly and royal jelly powders.
method.
This section applies to screening and detection of streptomycin and dihydrostreptomycin residues in royal jelly and royal jelly lyophilized powder.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
SN/T 2775 Commercial Food Testing Kit Evaluation Method
3 Method summary
This section uses acidic buffer to precipitate proteins in royal jelly, extract residual streptomycin and dihydrostreptomycin, extracts pass HLB column
Purification treatment. After purification, streptomycin and dihydrostreptomycin in the sample solution compete with streptomycin-encoded streptavidin for binding to streptavidin antibodies.
The antibodies were bound to microplates coated with antibodies against sheep anti-rabbit IgG. Remove unbound streptomycin, dihydrostreptomycin and
Enzymatically labelled streptomycin, then added to the substrate for color development, absorbance was measured using a microplate reader, and streptomycin and dihydrostreptomycin were sampled based on the absorbance values.
The content of prime.
4 Reagents and materials
Unless otherwise stated, all reagents were of analytical grade and water was a grade of water as specified in GB/T 6682.
4.1 Streptomycin test kit reference SN/T 2775 Organized by the CNCA Commercial Food Testing Kit Evaluation Expert Committee
price. Specific evaluation see Appendix A. The kit should be stored at 4°C~8°C with closed light. The dissolved enzyme label needs -15°C
Frozen. The kit consists of the following.
a) Pre-coated antibody 96-well plates. 12 x 8 wells.
b) Streptomycin standard solutions. 0 ng/mL, 0.5 ng/mL, 1.5 ng/mL, 4.5 ng/mL, 13.5 ng/mL, 40.5 ng/mL.
c) Streptomycinase Concentrate. According to the Streptomycin kit instructions, streptomycin enzyme markers can be prepared using dilution buffer.
Solution.
d) Diluent of the enzyme standard.
e) Sample diluent. According to the instructions in the streptomycin kit, dilute to a sample dilution working solution with deionized water.
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