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SN/T 4537.1-2016: Commercial kit method--Chloramphenicol--Test method Ⅰ
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| SN/T 4537.1-2016 | English | 139 |
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Commercial kit method--Chloramphenicol--Test method Ⅰ
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SN/T 4537.1-2016
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Basic data | Standard ID | SN/T 4537.1-2016 (SN/T4537.1-2016) | | Description (Translated English) | Commercial kit method--Chloramphenicol--Test method �� | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Word Count Estimation | 6,618 | | Date of Issue | 2016-08-23 | | Date of Implementation | 2017-03-01 | | Older Standard (superseded by this standard) | SN/T 2058-2008 | | Regulation (derived from) | State-Quality-Inspection-Certification (2016)438 | | Issuing agency(ies) | General Administration of Customs | SN/T 4537.1-2016: Commercial kit method--Chloramphenicol--Test method Ⅰ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Commercial kit detection method Chloramphenicol Method One)
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Commercial kit detection method
Chloramphenicol method one
Published on.2016-08-23
2017-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Entry and exit inspection and quarantine
Industry Standard
Commercial kit detection method
Chloramphenicol method one
China Standard Press Publishing
First edition in December.2017
Foreword
This part is the first part of the SN/T 4537 series of standards.
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. China Academy of Inspection and Quarantine, Beijing Qinbang Biotechnology Co., Ltd.
The main drafters of this section. Zou Mingqiang, Xue Qiang, Wang Yanfei, Wan Yuping, Luo Xiaoqin.
Commercial kit detection method
Chloramphenicol method one
1 Scope
This part of SN/T 4537 specifies the method for chloramphenicol enzyme-linked immunosorbent assay in food.
This section applies to the quantitative determination of chloramphenicol in chicken and shrimp by enzyme-linked immunoassay.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
SN/T 2775 Commercialized Food Testing Kit Evaluation Method
3 Principle
The antigen is pre-coated on the microplate of the microplate, and the chloramphenicol and the pre-coated conjugated antigen on the microporous strip compete with the chloramphenicol
The enzyme conjugate is developed with TMB substrate, and the absorbance of the sample is negatively correlated with the content of the residue chloramphenicol.
The chloramphenicol content of the sample can be obtained by the corresponding dilution factor.
4 reagents and materials
4.1 Chloramphenicol enzyme-linked immunosorbent assay kit
It is transported and stored at 2 °C ~ 8 °C. The product is valid for 12 months.
The kit reference SN/T 2775 was evaluated and evaluated by the National Accreditation Administration Committee for the Evaluation of Commercialized Food Testing Kits.
See Appendix A for the results. The kit consists of the following.
a) 96-well microtiter plate × 1 block, coated with coupled antigen;
b) Standard solution × 6 bottles, 1 mL/bottle, the concentration is 0 × 10-9, 0.025 × 10-9, 0.075 × 10-9, 0.3 × 10-9, 1.2 ×
10-9, 4.8×10-9;
c) high concentration standard. 1mL/bottle, the concentration is 100 × 10-9;
d) enzyme conjugate working solution, 7mL;
e) substrate liquid A, 7mL;
f) substrate liquid B, 7mL;
g) Stop solution, 7mL;
h) 20× concentrated washing solution, 40 mL;
i) 2 x concentrated complex solution, 50 mL.
4.2 ethyl acetate (analytical grade)
4.3 n-hexane (analytical grade)
4.4 Deionized water
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