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Commercial kit test method - Beta-lactams - Test method Ⅴ
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SN/T 4532.5-2017
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Basic data Standard ID | SN/T 4532.5-2017 (SN/T4532.5-2017) | Description (Translated English) | Commercial kit test method - Beta-lactams - Test method �� | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | G66 | Classification of International Standard | 71.040.30 | Word Count Estimation | 5,572 | Date of Issue | 2017-07-21 | Date of Implementation | 2018-03-01 | Quoted Standard | SN/T 2775 | Regulation (derived from) | National Quality Inspection (2017) No. 337 | Issuing agency(ies) | General Administration of Customs | Summary | This standard specifies the colloidal gold immunochromatographic assay for the determination of milk ��-lactams and tetracyclines. This standard is applicable to the qualitative detection of ��-lactam drugs and tetracycline residues in milk by colloidal gold immunochromatographic test strips. |
SN/T 4532.5-2017: Commercial kit test method - Beta-lactams - Test method Ⅴ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Commercial kit test method - Beta-lactams - Test method V
People's Republic of China entry and exit inspection and quarantine industry standards
Commercial kit detection method
--lactam method five
Released on.2017-07-21
2018-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This part is the fifth part of SN/T 4532 "Commercial kit detection method β-lactam".
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section is mainly drafted by. Jilin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Fujian Entry-Exit Inspection and Inspection
Epidemic Bureau, China Academy of Inspection and Quarantine, Beijing Boo Shide Biotechnology Co., Ltd.
The main drafters of this section. Nie Dandan, Luo Yanfei, Liu Jinhua, Zheng Jing, Liu Yang, Qu Hui, Peng Bo, Wang Ning, Lu Chunmei, Zou Mingqiang, Yan Jun.
Commercial kit detection method
--lactam method five
1 Scope
This part of SN/T 4532 specifies colloidal gold immunochromatographic assays for beta-lactams and tetracycline residues in milk.
This section applies to the qualitative detection of β-lactams and tetracycline residues in milk using colloidal gold immunochromatographic strips.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
SN/T 2775 Commercialized Food Testing Kit Evaluation Method
3 Principle
The method is based on colloidal gold immunoassay, the test strip is combined with the pad coated with the test antibody colloidal gold complex, and the test line is coated with the test substance.
The antigen, when the sample contains the test substance, competes with the test line to coat the antigen and binds to the gold standard complex, and the color of the detection line and the control line is deep.
A shallow comparison is made to achieve a qualitative judgment of the content of the analyte in the sample.
4 reagents and materials
4.1 β-lactam/tetracycline quadruple detection kit 1
). Refer to SN/T 2775 by the National Certification and Accreditation Administration for commercial food testing kits
The evaluation expert committee conducted an evaluation, and the evaluation results are shown in Appendix A.
1) Beijing Boo Shide Biotechnology Co., Ltd. product, given this information is for the convenience of users of this standard, does not mean recognition of the product.
If other products have the same effect, these equivalent products can be used.
4.2 Commercialized test strips, reaction cups and reagents should be stored at 0 °C ~ 7 °C, dry and protected from light.
4.3 The kit consists of the following.
a) test strips;
b) positive control;
c) Yin control.
5 instruments
5.1 Incubator. 56 ° C ± 1 ° C.
5.2 Reader.
5.3 Pipette. Adjustable range to 300μL.
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