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SN/T 4519-2016: Determination of ribavirin residue in foodstuffs of animal origin for export--LC-MS/MS method
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Determination of ribavirin residue in foodstuffs of animal origin for export--LC-MS/MS method
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SN/T 4519-2016
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Basic data | Standard ID | SN/T 4519-2016 (SN/T4519-2016) | | Description (Translated English) | Determination of ribavirin residue in foodstuffs of animal origin for export--LC-MS/MS method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Word Count Estimation | 22,286 | | Date of Issue | 2016-06-28 | | Date of Implementation | 2017-02-01 | | Regulation (derived from) | State-Quality-Inspection-Accredidation [2016] No.318 | | Issuing agency(ies) | General Administration of Customs | SN/T 4519-2016: Determination of ribavirin residue in foodstuffs of animal origin for export--LC-MS/MS method
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(Determination of the content of ribavirin residues in animal food by export - liquid chromatography - mass spectrometry/mass spectrometry)
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Residues of ribavirin in animal foods exported
Determination of liquid chromatography-mass spectrometry/mass spectrometry
LC-MS/MSmethod
Released.2016-06-28
2017-02-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. Publication of this document
The organization is not responsible for identifying these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Fujian Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Dongshan Entry and Exit Inspection and Quarantine of the People's Republic of China
Bureau, Xiamen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shaoxing Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Fujian Shengnong Development Unit
Co., Ltd.
The main drafters of this standard. Liu Zhengcai, Yang Fang, Pan Yingfen, Lin Yonghui, Chen Xudong, Xu Dunming, Ren Mingxing, Lu Xiaoling.
Residues of ribavirin in animal foods exported
Determination of liquid chromatography-mass spectrometry/mass spectrometry
1 Scope
This standard specifies the method for the determination of ribavirin residues in animal-derived foods by liquid chromatography-mass spectrometry/mass spectrometry.
This standard applies to the determination and confirmation of ribavirin residues in animal foods such as chicken, liver, kidney, egg, pork, squid and shrimp.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Method summary
The ribavirin metabolite remaining in the sample is hydrolyzed by the acid phosphatase into the ribavirin original drug, and the ribavirin remaining in the sample
The drug is extracted with trichloroacetic acid-acetic acid mixed solution, purified by phenylboric acid solid phase extraction cartridge, and determined by liquid chromatography-mass spectrometry/mass spectrometry.
Isotope internal standard method for quantification.
4 reagents and materials
Unless otherwise stated, the reagents used were of analytical grade and the water was the first grade water specified in GB/T 6682.
4.1 Methanol. Chromatographically pure.
4.2 Acetonitrile. chromatographically pure.
4.3 Formic acid. chromatographically pure.
4.4 Trichloroacetic acid.
4.5 Ammonia. The content is 25%~28%.
4.6 Ammonium acetate. chromatographically pure.
4.7 Acid phosphatase (fromwheatgerm, viability. ≥0.4 unit/mg, CAS number. 9001-77-8).
4.8 Acid Phosphatase Solution (100μg/mL). Accurately weigh 5mg of acid phosphatase (4.7), place it in a 50mL volumetric flask, and dissolve in water.
Solution and dilute to the mark, shake well, prepare 100 μg/mL of enzymatic hydrolysate, and store in 4 ° C ~ 8 ° C refrigerator.
4.9 Trichloroacetic acid solution (20g/L, pH 4.8). Weigh 20g of trichloroacetic acid, add about 950mL of water to dissolve it, adjust the pH with ammonia water to
4.8 (±0.1), add water to a volume of 1000 mL.
4.10 PBA phenylboronic acid solid phase extraction cartridge eluent, formic acid-water-methanol solution (volume ratio 2.8.90). accurately measure 2mL formic acid and
8 mL of water was added to 90 mL of methanol, and mixed for use.
4.11 Ammonium acetate buffer solution (2.0mol/L, pH4.8). Weigh 77.0g of ammonium acetate, add about 450mL of water to dissolve it, adjust with acetic acid
The pH was adjusted to 4.8 (±0.1), and water was added to dissolve to 500 mL.
4.12 Ammonium acetate buffer solution (0.25mol/L, pH 8.5). Weigh 9.06g of ammonium acetate, dissolve it with about 450mL of water, and adjust with ammonia.
The pH was adjusted to 8.5 (±0.1), and the volume was adjusted to 500 mL with water.
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