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Determination of clobetasol, beclomethasone and clobetasol propionate in cosmetics--HPLC-MS/MS method
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SN/T 4504-2016
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PDF similar to SN/T 4504-2016
Basic data Standard ID | SN/T 4504-2016 (SN/T4504-2016) | Description (Translated English) | Determination of clobetasol, beclomethasone and clobetasol propionate in cosmetics--HPLC-MS/MS method | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | Y42 | Word Count Estimation | 10,168 | Date of Issue | 2016-06-28 | Date of Implementation | 2017-02-01 | Regulation (derived from) | State-Quality-Inspection-Accredidation [2016] No.318 | Issuing agency(ies) | General Administration of Customs |
SN/T 4504-2016: Determination of clobetasol, beclomethasone and clobetasol propionate in cosmetics--HPLC-MS/MS method ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of clobetasol, beclomethasone and clobetasol propionate in cosmetics - HPLC-MS/MS method
People's Republic of China Entry - Exit Inspection and Quarantine Industry Standard
Export cosmetics in clobetasil, beclomethasone, chlorine times
Determination of his propionate liquid chromatography-mass spectrometry /
Mass spectrometry
Determination ofclobetasol, beclomethasoneandclobetasol
propionateincosmetics-HPLC-MS/MS method
2016-06-28 released
2017-02-01 implementation
People's Republic of China
State Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuer of this document does not assume responsibility for the identification of these patents.
This standard is proposed and approved by the National Certification and Accreditation Administration Committee.
The drafting unit of this standard. China Inspection and Quarantine Science Research Institute.
The main drafters of this standard. Bai Hua, Li Haiyu, Zhang Qing, Ma Qiang, Mengxian Shuang, Wang Wan, Guo Xiangyu, Wang Zhijuan.
Export cosmetics in clobetasil, beclomethasone, chlorine times
Determination of his propionate liquid chromatography-mass spectrometry /
Mass spectrometry
1 Scope
This standard specifies the liquid, mass/selenium,
Mass spectrometry.
This standard applies to the water, cream exports of cosmetics in the sales of chlorpheniramine, beclomethasone, clobetasone propionate test.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 principle
Directly to take water cream cream cosmetics, dissolved with methanol, vortex mixing, and then ultrasonic extraction of hormone drugs, centrifugal supernatant, the
Solid phase extraction column purification, reverse phase high performance liquid chromatography tandem mass spectrometry, external standard quantitative.
4 reagents and materials
Unless otherwise specified, all reagents are of analytical grade and water is a primary water specified in GB/T 6682.
4.1 acetonitrile. pure chromatography.
4.2 Methanol. Chromatographic purity.
4.3 Formic acid. Analytical purity.
4.4 Chlorperethritol standard material. C22H28ClFO4, CAS No. 25122-41-2, purity ≥ 98%.
4.5 beclomethasone standard substance. C22H29ClO5, CAS No. 4419-39-0, purity 98%.
4.6 Chlorperethritol propionate Reference material. C25H32ClFO5, CAS No. 25122-46-7, purity 99%.
4.7 standard stock solution. accurate reference standard substances clobetasil (4.4), beclomethasone (4.5), clobetasil propionate (4.6) each 50mg
(Accurate to 0.1mg) in 50mL brown volumetric flask, respectively, with methanol dissolved and fixed to the scale, prepared into a concentration of 1000mg/L
Standard single standard stock solution, stored at 4 ° C.
4.8 standard mixed working solution. take the standard stock solution (4.7) 1mL mixed with methanol fixed in the 100mL volumetric flask, made of concentration
10mg/L standard mixed stock solution, stored at 4 ℃, temporary use with the initial mobile phase solution diluted to 100ng/mL, 50ng/mL,
20ng/mL, 10ng/mL, 5ng/mL, 2ng/mL, 1ng/mL and other series of standard mixed working solution for the production of standard
curve.
4.9 OasisHLB solid phase extraction column or equivalent. 60mg, 3mL.
4.10 Microporous membrane. organic film, pore size 0.2μm.
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