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SN/T 4280.1-2015 English PDF

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SN/T 4280.1-2015: DNA barcoding identification of Calliphora vicina Robineau. Desvoidy, 1830 at the frontier port
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Standard similar to SN/T 4280.1-2015

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Basic data

Standard ID SN/T 4280.1-2015 (SN/T4280.1-2015)
Description (Translated English) DNA barcoding identification of Calliphora vicina Robineau. Desvoidy, 1830 at the frontier port
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard C62
Classification of International Standard 07.080
Word Count Estimation 16,179
Date of Issue 2015-05-26
Date of Implementation 2016-01-01
Quoted Standard GB 19489; SN/T 1876; SN/T 2752.4; WS/T 230
Regulation (derived from) State Quality-Inspection-Accreditation [2015] No.224
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the Calliphora vicina of DNA barcoding process and result judgment. This standard applies to Calliphora vicina adults (including adult limb insufficiency), and the work of pupae, larvae, eggs, etc. DNA barcoding identification.

SN/T 4280.1-2015: DNA barcoding identification of Calliphora vicina Robineau. Desvoidy, 1830 at the frontier port


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(Border crossings Calliphora vicina DNA barcoding method) People's Republic of China Entry-Exit Inspection and Quarantine Standards Border crossings Calliphora vicina DNA barcoding method Issued on. 2015-05-26 2016-01-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This section drafted in accordance with GB/T 1.1-2009 given rules. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section was drafted by. People's Republic of China Guangdong Entry Inspection and Quarantine Bureau, People's Republic of China Hebei Entry-Exit Inspection and Quarantine Bureau. The main drafters of this section. Qiu Deyi, Yueqiao Yun, Zhang Xianchen, Liu Xing, Nie Weizhong, Chen Jian, Wei Xiaoya, Wu scalability, Hu Jia. Border crossings Calliphora vicina DNA barcoding method

1 Scope

SN/T 4280 provisions of this part of the Calliphora vicina of DNA barcoding process and result judgment. This section applies to Calliphora vicina adults (including adult limb insufficiency), and pupae, larvae, eggs, etc. DNA barcoding identification jobs.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB 19489 General requirements for laboratory biosafety SN/T 1876 medical vectors specimen collection, production and preservation regulations SN/T 2752.4 specification of self-protection of the health and quarantine personnel - Part 4. Laboratory personnel Applications WS/T 230 clinical diagnostic polymerase chain reaction (PCR) technology

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Calliphora vicina CalliphoravicinaRobineau-Desvoidy, 1830 Calliphoridae, Calliphoridae spp. Because the abdomen cyan, commonly known as "blueblowfly" or "bluebottle", because of its orange-red cheek, so Chinese name Calliphora vicina, as an important medium for biological and forensic insects. 3.2 DNA barcoding DNAbarcode To represent the living body of the species, the standard, there is enough variation, and relatively easy to amplify short DNA fragments. DNA barcoding Species identification code has become an important tool in arthropods, especially insects, generally refers to mitochondrial COI (cytochrome C Oxidase subunit I of the standard fragment 658bp) on. 3.3 DNA barcoding technology DNAbarcodingidentificationtechnology A use of DNA barcoding gene fragment species identification and phylogenetic studies of the relationship between technology, based on gene amplification And on the basis of sequence alignments, it has not species developmental status, sex and morphological characteristics of the complete absence of limits to reduce the morphological classification Dependent experts, is not affected by subjective factors, high accuracy, using the Internet can achieve data sharing and so on. 3.4 Blank blankcontrol Starting from the genomic DNA extraction step to PCR amplification and PCR product detection to the whole process, not a member of any medical vector insects Blank organization, in parallel with the other insects tested to operate in order to observe the experiment is in a normal state. There should be no blank PCR product Strip was produced.

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