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SN/T 4273-2015: Real time PCR detection methods of Banna virus at ports
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| SN/T 4273-2015 | English | 139 |
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Real time PCR detection methods of Banna virus at ports
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SN/T 4273-2015
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Basic data | Standard ID | SN/T 4273-2015 (SN/T4273-2015) | | Description (Translated English) | Real time PCR detection methods of Banna virus at ports | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Classification of International Standard | 11.020 | | Word Count Estimation | 6,675 | | Date of Issue | 2015-05-26 | | Date of Implementation | 2016-01-01 | | Regulation (derived from) | State Quality-Inspection-Accreditation [2015] No.224 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the real-time fluorescent RT-PCR method to detect viruses Banna border crossings, including specimen collection, handling, inspection procedures, methods and results determined. This standard applies to border crossings Banna virus testing laboratory. | SN/T 4273-2015: Real time PCR detection methods of Banna virus at ports---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Real time PCR detection methods of Banna virus at ports
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Fluorescent PCR detection method for border virus of border port
Released on.2015-05-26
2016-01-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Sichuan Entry-Exit Inspection and Quarantine
Bureau, Xiamen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, China Academy of Inspection and Quarantine.
The main drafters of this standard. Hong Wei, Ding Guoyun, Sun Wei, Zhou Yanrong, Shi Yongxia, Zheng Yi, Huang Jicheng, Liu Yang, Dai Jun, Su Shuikuan, He Wei,
Sun Xiaohong, Xing Luqin, Li Xiaobo, Zhong Yuqing, Xiang Dapeng, Guo Boxuan.
Fluorescent PCR detection method for border virus of border port
1 Scope
This standard specifies the real-time fluorescent RT-PCR detection method for border virus at border port, including specimen collection, processing, inspection procedures,
Method and result determination.
This standard applies to laboratory testing of border virus at border ports.
2 Abbreviations
The following abbreviations apply to this document.
Real-time fluorescent RT-PCR. real-time fluorescent reverse transcription-polymerase chain reaction
Ct value. number of cycles experienced when the fluorescence signal in each reaction tube reaches a set threshold
RNA. ribonucleic acid
FAM. FAM fluorescent dye, a fluorescent reporter group
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Bannavirus
Bannavirus (BAV) is a 12-segment double-stranded RNA virus, an arbovirus that is mainly transmitted by mosquitoes and can be cited.
Human fever and viral encephalitis are present, and the virus is currently distributed mainly in China and Indonesia. Banna virus was observed under electron microscope
Icosahedral spherical particles with a fiber surface on the surface, between 60nm and 70nm in diameter. The genome of the virus consists of 12 segments of double strands (dsR-
NA) Composition, with a total length of approximately 21,000 bp.
3.2
Real-time fluorescence PCR real-timefluorescence PCR
The real-time fluorescent PCR method is based on conventional PCR and adds a fluorescent reporting system that reflects the PCR amplification process.
The monitoring system receives the fluorescent signal and determines the template amplification of the target gene in each reaction tube in real time.
4 main equipment
The main instruments used in this method include fluorescence quantitative PCR instrument, ultra-clean workbench, class II biosafety cabinet, autoclave, low temperature
Speed centrifuge (maximum centrifugal force.20000 × g), glass grinder, vortex shaker, refrigerator (4 ° C, -20 ° C, -70 ° C or liquid nitrogen tank), shift
Liquid etc.
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