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SN/T 4235-2015 English PDF

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SN/T 4235-2015: Quarantine protocol for swine hepatitis E
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PDF similar to SN/T 4235-2015


Standard similar to SN/T 4235-2015

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Basic data

Standard ID SN/T 4235-2015 (SN/T4235-2015)
Description (Translated English) Quarantine protocol for swine hepatitis E
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B41
Word Count Estimation 13,168
Date of Issue 2015-05-26
Date of Implementation 2016-01-01
Regulation (derived from) State Quality-Inspection-accreditation [2015] No.224
Issuing agency(ies) General Administration of Customs

SN/T 4235-2015: Quarantine protocol for swine hepatitis E

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for swine hepatitis E People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Swine hepatitis hepatitis quarantine technical specification Released on.2015-05-26 2016-01-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shanghai Entry-Exit Inspection and Quarantine Bureau, Beijing Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shanghai Animal Disease Prevention and Control Center. The main drafters of this standard. Yu Haiqiong, Zhang Qiang, Qiao Caixia, Luo Changbao, Zhao Wei, Cai Xianquan, Liu Peihong, Zhou Jinping, Li Shuqing, Wu Xiaowei. Swine hepatitis hepatitis quarantine technical specification

1 Scope

This standard specifies the clinical diagnosis of porcine hepatitis E, enzyme-linked immunosorbent assay, real-time fluorescent polymerase chain reaction detection technology. This standard applies to the diagnosis and epidemiological investigation of swine hepatitis E.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 18088 entry and exit animal quarantine sampling GB/T 19489 General requirements for laboratory biosafety

3 Abbreviations

The following abbreviations apply to this document. HEV. Hepatitis E virus BSA. Bovine Serum Albumin HRP. horseradish peroxidase OD value. optical density value ELISA. enzyme-linked immunosorbent assay Fluorescence RT-PCR. real-time fluorescent polymerase chain reaction

4 reagents

4.1 HEV positive control serum. 4.2 HEV negative control serum. 4.3 HEV antigen. 4.4 Bovine serum albumin. 4.5 HRP-labeled rabbit anti-porcine IgG. 4.6 0.01 mol/L PBS buffer. 4.7 Antigen dilution (see Appendix A). 4.8 Washing liquid (see Appendix A). 4.9 Tween-20. 4.10 Blocking solution (see Appendix A). 4.11 Reagent (see Appendix A). 4.12 Stop solution (see Appendix A). 4.13 TRIZOL. 4.14 chloroform.

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