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SN/T 4179-2015: Detection and identification of Narcissus degeneration virus
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Detection and identification of Narcissus degeneration virus
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SN/T 4179-2015
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Basic data | Standard ID | SN/T 4179-2015 (SN/T4179-2015) | | Description (Translated English) | Detection and identification of Narcissus degeneration virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 12,196 | | Date of Issue | 2015-02-09 | | Date of Implementation | 2015-09-01 | | Quoted Standard | SN/T 2122 | | Regulation (derived from) | State-Quality-Inspection-Accreditation [2015] 59 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the Narcissus degeneration virus serology, molecular biology quarantine and identification methods. This standard applies to Amaryllidaceae Narcissus degeneration and other host plants of quarantine virus identification. | SN/T 4179-2015: Detection and identification of Narcissus degeneration virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Narcissus degradation quarantine and identification of virus)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Narcissus degradation quarantine and identification of viruses
Issued on. 2015-02-09
2015-09-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Xiamen, People's Republic of China Exit Inspection and Quarantine, People's Republic of China Fujian Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. Lin Shiming, Shen Jianguo, Liao Furong, Fang Zhipeng Wu Yuan, Chen Qing, Chen Yun, Peng Ying Huang.
Narcissus degradation quarantine and identification of viruses
1 Scope
This standard specifies the Narcissus degeneration virus serology, molecular biology quarantine and identification methods.
This standard applies to Amaryllidaceae Narcissus degeneration and other host plants of quarantine virus identification.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 2122 entry and exit quarantine of plants and plant products sampled
3 Narcissus degradation virus basic information
Chinese name. Narcissus degradation virus
Name. Narcissusdegenerationvirus
Abbreviation. NDV
Belonging to potato virus Y Branch (Potyviridae), potato virus Y genus (Potyvirus) members.
Transmission. bulbs transmission, mechanical transmission.
NDV For additional information, see Appendix A.
4 principle of the method
Narcissus degeneration virus serological, molecular biological characteristics is the main basis of the development of methods for identification of quarantine.
5 equipment and appliances
Electronic balance (1/1000g), high-speed refrigerated centrifuge, PCR instrument, electrophoresis, horizontal electrophoresis tank gel imaging analysis system, pH meter,
Various measurement ranges adjustable pipette (1000μL, 200μL, 100μL, 20μL, 10μL, 2μL), PCR reaction tubes, no RNaseEppen-
dorf centrifuge tube (1.5mL), a mortar, a microwave, a magnetic stirrer, constant temperature water bath, autoclaves, low-temperature ultra-low temperature freezer and refrigerator.
6 Detection and Identification
6.1 Sampling Inspection
Sampling method according to SN/T 2122 given sample.
6.2 antigen capture ELISA test (PTA-ELISA)
According to the method given in Appendix B PTA-ELISA test. A known virus isolates as positive control, healthy plant tissues as Yin
Of the control sample extraction buffer as the blank.
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