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SN/T 4141-2015 English PDF

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SN/T 4141-2015: Determination of glucocorticoid residues in livestock and livestock products. Enzyme-linked immunosorbent assay
Status: Valid
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PDF similar to SN/T 4141-2015


Standard similar to SN/T 4141-2015

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Basic data

Standard ID SN/T 4141-2015 (SN/T4141-2015)
Description (Translated English) Determination of glucocorticoid residues in livestock and livestock products. Enzyme-linked immunosorbent assay
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard X22
Classification of International Standard 67.120
Word Count Estimation 9,996
Date of Issue 2015-02-09
Date of Implementation 2015-09-01
Quoted Standard GB/T 6682
Regulation (derived from) State-Quality-Inspection-Accreditation [2015] 59
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the livestock and livestock products dexamethasone, prednisolone, dexamethasone fluorine, isoflurane prednisolone, betamethasone, triamcinolone and Flumetholon and other glucocorticoids residues in sampling, sample preparation and enzyme-linked immunosorbent assay method. This standard applies to livestock (cattle, pigs, sheep and other visceral tissue and muscle tissue), milk and animal urine, dexamethasone, prednisolone, dexamethasone fluorine, isoflurane prednisolone, betamethasone, go triamcinolone and fluorine to Long and other glucocorticoids residues screening test.

SN/T 4141-2015: Determination of glucocorticoid residues in livestock and livestock products. Enzyme-linked immunosorbent assay


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Livestock and livestock products in glucocorticoid Residues enzyme-linked immunosorbent assay) People's Republic of China Entry-Exit Inspection and Quarantine Standards Livestock and livestock products glucocorticoid Residues Detection ELISA Issued on. 2015-02-09 2015-09-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Han Wei, Wang win, Yuan Chen Gang, Zhu Jian. Livestock and livestock products glucocorticoid Residues Detection ELISA

1 Scope

This standard specifies the livestock and livestock products dexamethasone, prednisolone, dexamethasone fluorine, isoflurane prednisolone, betamethasone, triamcinolone and fluorine Sampling, sample preparation and enzyme-linked immunoassay Miron and other glucocorticoids residues. This standard applies to livestock (cattle, pigs, sheep and other visceral tissue and muscle tissue), milk and animal urine, dexamethasone, prednisolone, Fluorine dexamethasone, isoflurane prednisolone, betamethasone, triamcinolone and Flumetholon and other glucocorticoids residues screening test.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis

3 reduction and preservation of the sample points

Reduction of sub-samples 3.1 3.1.1 Tissue samples. From the entire sample (including milk) in a representative sample is taken, remove the fat, full minced, mix, and dividing by quartering Not less than 500g sample. 3.1.2 animal urine. the urine collected from the animal rearing site, thoroughly mixed, after clarification in a clean airtight container, a sample of not less than 50mL. 3.2 sample container Samples should be installed in a suitable clean, dry container, in order to maintain the integrity and traceability of the sample. Can be used polyethylene plastic containers, glass Glass containers and other inert material container (not allowed to use a rubber container) containing the sample, then placed in a large clean container sealed for shipment. 3.3 Sample seals Should be affixed on the outside of each sample container labels indicate on the label the name of the sample, sample number, batch number, date of sampling, sampling locations, Heap bit, sampling staff, by the sampling officer or official who sealed. 3.4 Sample Storage Laboratory samples should be immediately delivered within 48h after sampling 3.4.1 below -18 ℃ frozen and stored, 0 ~ 5 ℃ condition. 3.4.2 Sampling and sample preparation process, should prevent sample contamination or the occurrence of the change of residue content.

4 Determination

4.1 Principle Determination of the basis of this method is a competitive enzyme immunoassay. Microplate coated with sheep anti-rabbit IgG antibody, specific anti-join

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