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SN/T 4136-2015 English PDF

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SN/T 4136-2015: Determination of aloin in aloe powder for export. HPLC method
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Basic data

Standard ID SN/T 4136-2015 (SN/T4136-2015)
Description (Translated English) Determination of aloin in aloe powder for export. HPLC method
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard X26
Classification of International Standard 67.080.20
Word Count Estimation 7,746
Date of Issue 2015-02-09
Date of Implementation 2015-09-01
Quoted Standard GB/T 6682
Regulation (derived from) State-Quality-Inspection-Accreditation [2015] 59
Issuing agency(ies) General Administration of Customs
Summary This standard specifies a high performance liquid chromatographic method in the export of aloe vera powder saponin content. This standard applies to Aloe vera raw material consumption of aloe powder quantitative determination of aloin content.

SN/T 4136-2015: Determination of aloin in aloe powder for export. HPLC method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Aloe vera powder exports glycosides content by HPLC) People's Republic of China Entry-Exit Inspection and Quarantine Standards Determination of the export of aloe vera powder saponin content High Performance Liquid Chromatography Issued on. 2015-02-09 2015-09-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Institutions do not assume the responsibility to identify these patents. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Anhui Entry-Exit-Entry Inspection and People's Republic of China Yunnan Quarantine Bureau. The main drafters. Zhang Ping, Pengyun Xia, Yu Xiaofeng, Han Fang, Zhu Xu Meng, Yao Jian, Hu Yanyun. Determination of the export of aloe vera powder saponin content High Performance Liquid Chromatography

1 Scope

This standard specifies a high performance liquid chromatographic method in the export of aloe vera powder saponin content. This standard applies to Aloe vera raw material consumption of aloe powder quantitative determination of aloin content.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 6682 analytical laboratory use specifications and test methods (ISO 3696. 1987, MOD)

3 Method summary

Sample directly with aqueous methanol ultrasonic extraction, with the use of a diode array UV detector or liquid chromatograph, a standard curve External standard.

4 Reagents and materials

Unless otherwise noted, all reagents were of analytical grade water as a water GB/T 6682 stipulated. 4.1 Methanol. chromatographically pure. 4.2 acetic acid. pure class distinctions. 4.3 Aloin standards. C20H20O8, CAS No. 1415-73-2, purity greater than or equal to 98%. 4.4 Aloin standard stock solution. weigh accurately 10.0mg (accurate to 0.1mg) Aloin standards in 10mL volumetric flask with methanol Dissolve and dilute to the mark, formulated at a concentration of 1mg/mL standard stock solution, -18 ℃ under dark save for one week. 4.5 microporous membranes. organic, 0.45μm. 5. Apparatus 5.1 High performance liquid chromatograph. with or UV diode array detector. 5.2 Analysis of balance. a sense of the amount of 0.1mg. 5.3 ultrasonic cleaning. 5.4 centrifuge. speed of not less than 4000r/min.

6 measuring step

Sample preparation before 6.1 Aloe vera powder was pulverized into a powder samples evenly after accurately weighed 0.5g (accurate to 0.1mg), a 50mL volumetric flask.

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