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SN/T 4079-2014 English PDF

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SN/T 4079-2014: Detection and identification of Cactodera Krall ＆ Krall1978
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Basic data

Standard ID SN/T 4079-2014 (SN/T4079-2014)
Description (Translated English) Detection and identification of Cactodera Krall �� Krall1978
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 07.080
Word Count Estimation 34,380
Date of Issue 12/1/2014
Date of Implementation 5/1/2015
Quoted Standard GB/T 24829; SN/T 1157; SN/T 1158; SN/T 1723.1; SN/T 1723.2
Regulation (derived from) State-Quality-Inspection-accreditation [2014] 614
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the method of entry-exit inspection and identification of plant quarantine in the genus Opuntia cyst nematodes. This standard applies to immigration plant quarantine of plants, soil and medium cactus quarantine and identification o

SN/T 4079-2014: Detection and identification of Cactodera Krall ＆ Krall1978

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(Cyst nematode genus Opuntia quarantine and identification methods) People's Republic of China Entry-Exit Inspection and Quarantine Standards Cactus cyst nematode quarantine and identification methods Issued on. 2014-11-19 2015-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Tianjin People's Republic of China Entry-Exit Inspection and Quarantine Bureau, Shenzhen People's Republic of China Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Wang Jincheng, Guo Jingze, Longhai, Huang Guoming, Roca Feng, Liu Fang, Liuyue Ting, Liu Peng. Cactus cyst nematode quarantine and identification methods

1 Scope

This standard specifies the method of entry-exit inspection and identification of plant quarantine in the genus Opuntia cyst nematodes. This standard applies to immigration plant quarantine of plants, soil and medium cactus quarantine and identification of cyst nematode.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 24829 Burr nematode genera (mass poisoning species) identification of quarantine SN/T 1157 entry plant quarantine rules seedlings SN/T 1158 entry bonsai plant quarantine rules SN/T 1723.1 white potato nematode quarantine and identification methods SN/T 1723.2 quarantine and identification of Globodera

3 PRINCIPLE OF THE METHOD

Chinese name. genus Opuntia cysts Name. CactoderaKral Taxonomic position. door nematode (Nematoda), side tail gland Gang (Secernentea), Tylenchida (Tylenchida), link suborder (Hoplo- laimina), link superfamily (Hoplolaimoidea), iso Picco (Heteroderidae), iso Piya Ke (Heteroderinae). Opuntia genus cysts There are currently 13 species. Cysts shape, the diameter of the membrane pores, the presence or absence of surface features protruding vulva, egg shells, child age 2 Body length and tail and transparent tail length is mainly based on morphological characteristics of the genus classification and identification; their biological characteristics, host range, geographical Distribution, damage symptoms, transmission and molecular biological characteristics can be determined as a secondary basis. Cactus cyst nematode host range, geographical Distribution, and transmission damage symptoms, see Appendix A, the membrane pore shape in Appendix B. 4 instruments, equipment and reagents 4.1 apparatus and appliances Optical microscopes, stereo microscopes, incubators, floating barrels, refrigerator, hot plates, standard set of sieves (20 mesh to 500 mesh), beaker (2000mL), Dryer, brush, brush, PCR instrument, electrophoresis, electrophoresis tank, gel imaging system. 4.2 Reagents Alcohol, glycerin, 40% formaldehyde, phenol milk oil, clove oil, hydrogen peroxide, neutral gum, magnesium sulfate, paraffin, rTaqDNA polymerase, dNTP, primer and the like. 5-spot quarantine Observe whether the aerial parts of seedlings and roots organizations dysplasia symptoms, and with or without soil media. Key Select floor

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