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(Rice bacterial leaf spot pathogen, citrus canker, microarray screening cabbage black rot bacteria)
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SN/T 4075-2014
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Basic data | Standard ID | SN/T 4075-2014 (SN/T4075-2014) | | Description (Translated English) | (Rice bacterial leaf spot pathogen, citrus canker, microarray screening cabbage black rot bacteria) | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Date of Issue | 12/1/2014 | | Date of Implementation | 5/1/2015 | | Regulation (derived from) | State-Quality-Inspection-accreditation [2014] 614 | | Issuing agency(ies) | General Administration of Customs | SN/T 4075-2014: (Rice bacterial leaf spot pathogen, citrus canker, microarray screening cabbage black rot bacteria)
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(Rice bacterial leaf spot pathogen, citrus canker, microarray screening cabbage black rot bacteria)
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Rice bacterial leaf spot pathogen, citrus canker,
Gene chip screening method for cabbage black rot
ScreenofXanthomonasoryzaepv.oryzicola, Xanthomonasaxonopodispv.citri
Released on.2014-11-15
2015-05-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Entry and exit inspection and quarantine
Industry Standard
Rice bacterial leaf spot pathogen, citrus canker,
Gene chip screening method for cabbage black rot
China Standard Press Publishing
First edition in January.2016
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Institute of Inspection and Quarantine, Chinese People
Republic of Yili Entry and Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Long Hai, Li Yinong, Li Fangrong, Ling Xingyuan, Yu Daojian, Zheng Yi, Feng Jianjun, Gan Yike.
Rice bacterial leaf spot pathogen, citrus canker,
Gene chip screening method for cabbage black rot
1 Scope
This standard specifies the bacterial pathogen of rice (Xranthomonasoryzaepv.oryzicola; in the entry and exit phytosanitary;
Xooc), citrus canker (X.axonopodispv.citri; Xac) and cabbage black rot (X.campestrispv. campestris;
Xcc) gene chip screening method.
This standard is applicable to rice bacterial leaf spot pathogens, citrus cankers and plants in in and out of rice and its products.
And gene chip screening of Brassica oleracea in cruciferous plants and their products.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB 19489 General requirements for laboratory biosafety
Method for detecting SN/T 2372 rice bacterial blight fungus and rice bacterial leaf spot pathogen
SN/T 2622 Quarantine and identification method for citrus canker
3 Terms, definitions and abbreviations
3.1 Terms and definitions
The following terms and definitions apply to this document.
3.1.1
Gene chip genearray
The nucleic acid detection probe is spotted on the solid support in an ordered array format, and hybridized with the corresponding sample at a specific temperature.
A high-throughput technique for the qualitative analysis of nucleic acid species in samples.
3.1.2
Substrate substrate
The matrix used to immobilize the probe in the gene chip, usually prepared by chemical modification using standard "slides or other solid carriers"
Made.
3.1.3
Gene chip probe DNAmicroarrayprobe
A nucleic acid molecule immobilized on the surface of a substrate and complementary to the sample DNA, used to detect DNA information of the sample, and multi-purpose oligo
The nucleotide fragment is used as a probe.
3.1.4
Positioning probe positivepositionprobe
Is an oligonucleotide that is not related to the gene to be detected, and displays a signal by hybridizing with a complementary strand of the labeled localization probe for determining the spot
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