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SN/T 4074-2014
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Basic data Standard ID | SN/T 4074-2014 (SN/T4074-2014) | Description (Translated English) | (Oats quarantine all pathogen identification methods) | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | B16 | Word Count Estimation | 12,115 | Date of Issue | 12/1/2014 | Date of Implementation | 5/1/2015 | Regulation (derived from) | State-Quality-Inspection-accreditation [2014] 614 | Issuing agency(ies) | General Administration of Customs |
SN/T 4074-2014: (Oats quarantine all pathogen identification methods)---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Oats quarantine all pathogen identification methods)
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Method for quarantine and identification of oat total rot
Released on.2014-11-19
2015-05-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. Publication of this document
The organization is not responsible for identifying these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Hunan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, China Academy of Inspection and Quarantine, and the Chinese People
And Guangdong Entry-Exit Inspection and Quarantine Bureau, Huangpu Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Entry and Exit Inspection of the People's Republic of China
Quarantine Bureau, Xinjiang Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Mo Zhen, Gong Qiang, Tian Wei, Wang Wei, Bai Xue, Jiang Jinlin, Zhou Huiping, Peng Yu, Tan Jianxi, Zhong Guoqiang, Zhang Guiming,
Zhang Xianglin, Zhu Jinguo.
Method for quarantine and identification of oat total rot
1 Scope
This standard specifies the quarantine and identification methods for the whole pathogen of the oats (Gaeumannomyces graminisvar. avenae).
This standard is applicable to the detection of oat total pathogens in grasses, herbaceous plants, seeds and mixed plant residues.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
SN/T 1809 In and out plant seed quarantine regulations
SN/T 2122 Quarantine sampling of inbound and outbound plants and plant products
SN/T 2589 Plant Pathogenic Fungus Detection Specification
3 oat total pathogens basic information
Scientific name. Gaemannomydesgraminisvar.avenae(EMTurner)Dennis
Classification status. Ascomycotina, Pyrrenomycetes, Sphaeriales, inter-shell
Diaporthaceae, Gaeumannomyces, Gaeumannomyces gramims.
Route of transmission. The pathogen is overwintered in the soil by the mycelium in the diseased plant tissue or the diseased body (over summer). The entire growth period of grass and its various
The parts can be infected.
See Appendix A for additional information on oat total pathogens.
4 Principle of the method
On-site inspection based on plant pathology of plants, and using morphological characteristics, molecular biology and pathogenicity determination for plant seedlings, planting
Determination of oat total pathogens in seeds and mixed plant residues.
5 instruments and consumables
5.1 Instrumentation
PCR instrument, ultra-clean workbench, stereo microscope, sterilizer, ice machine, nucleic acid protein analyzer, high-speed refrigerated centrifuge, desktop small separation
Heart machine, ultra-low temperature refrigerator, artificial climate incubator, conventional refrigerator, vortex shaker, micro-sampler, electrophoresis, gel imaging system, enzyme
Standard instrument.
5.2 main reagent
All reagents were of analytical grade unless otherwise stated. PCR buffer, dNTPs (dATP, dTTP, dCTP, dGTP), Taq
DNA polymerase, PDA medium, PDB medium, 0.001% Tween 20-phosphate buffer, see Appendix B.
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