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SN/T 4074-2014 English PDF

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SN/T 4074-2014: (Oats quarantine all pathogen identification methods)
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Basic data

Standard ID SN/T 4074-2014 (SN/T4074-2014)
Description (Translated English) (Oats quarantine all pathogen identification methods)
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B16
Word Count Estimation 12,115
Date of Issue 12/1/2014
Date of Implementation 5/1/2015
Regulation (derived from) State-Quality-Inspection-accreditation [2014] 614
Issuing agency(ies) General Administration of Customs

SN/T 4074-2014: (Oats quarantine all pathogen identification methods)

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(Oats quarantine all pathogen identification methods) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Method for quarantine and identification of oat total rot Released on.2014-11-19 2015-05-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. Publication of this document The organization is not responsible for identifying these patents. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Hunan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, China Academy of Inspection and Quarantine, and the Chinese People And Guangdong Entry-Exit Inspection and Quarantine Bureau, Huangpu Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Entry and Exit Inspection of the People's Republic of China Quarantine Bureau, Xinjiang Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Mo Zhen, Gong Qiang, Tian Wei, Wang Wei, Bai Xue, Jiang Jinlin, Zhou Huiping, Peng Yu, Tan Jianxi, Zhong Guoqiang, Zhang Guiming, Zhang Xianglin, Zhu Jinguo. Method for quarantine and identification of oat total rot

1 Scope

This standard specifies the quarantine and identification methods for the whole pathogen of the oats (Gaeumannomyces graminisvar. avenae). This standard is applicable to the detection of oat total pathogens in grasses, herbaceous plants, seeds and mixed plant residues.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. SN/T 1809 In and out plant seed quarantine regulations SN/T 2122 Quarantine sampling of inbound and outbound plants and plant products SN/T 2589 Plant Pathogenic Fungus Detection Specification

3 oat total pathogens basic information

Scientific name. Gaemannomydesgraminisvar.avenae(EMTurner)Dennis Classification status. Ascomycotina, Pyrrenomycetes, Sphaeriales, inter-shell Diaporthaceae, Gaeumannomyces, Gaeumannomyces gramims. Route of transmission. The pathogen is overwintered in the soil by the mycelium in the diseased plant tissue or the diseased body (over summer). The entire growth period of grass and its various The parts can be infected. See Appendix A for additional information on oat total pathogens.

4 Principle of the method

On-site inspection based on plant pathology of plants, and using morphological characteristics, molecular biology and pathogenicity determination for plant seedlings, planting Determination of oat total pathogens in seeds and mixed plant residues.

5 instruments and consumables

5.1 Instrumentation PCR instrument, ultra-clean workbench, stereo microscope, sterilizer, ice machine, nucleic acid protein analyzer, high-speed refrigerated centrifuge, desktop small separation Heart machine, ultra-low temperature refrigerator, artificial climate incubator, conventional refrigerator, vortex shaker, micro-sampler, electrophoresis, gel imaging system, enzyme Standard instrument. 5.2 main reagent All reagents were of analytical grade unless otherwise stated. PCR buffer, dNTPs (dATP, dTTP, dCTP, dGTP), Taq DNA polymerase, PDA medium, PDB medium, 0.001% Tween 20-phosphate buffer, see Appendix B.

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