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SN/T 4073-2014 English PDF

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SN/T 4073-2014: Detection and identification of Xanthomonas campestris pv.mangiferaeindicae
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SN/T 4073-2014English289 Add to Cart 3 days [Need to translate] Detection and identification of Xanthomonas campestris pv.mangiferaeindicae Valid SN/T 4073-2014

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Basic data

Standard ID SN/T 4073-2014 (SN/T4073-2014)
Description (Translated English) Detection and identification of Xanthomonas campestris pv.mangiferaeindicae
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 07.080
Word Count Estimation 11,124
Date of Issue 12/1/2014
Date of Implementation 5/1/2015
Regulation (derived from) State-Quality-Inspection-accreditation [2014] 614
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the method for inspection and identification mango bacterial black spot pathogen Xanthomonas campestris pv.mangiferaeindicae of. This standard applies to the entry and exit of the mango fruit, leaves, branches, organizations may ca

SN/T 4073-2014: Detection and identification of Xanthomonas campestris pv.mangiferaeindicae

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Mango bacterial black spot disease rapid detection method) People's Republic of China Entry-Exit Inspection and Quarantine Standards Mango bacterial black spot disease rapid tests Issued on. 2014-11-19 2015-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Fujian Entry-Exit Inspection and Quarantine Xiamen People's Republic of China Bureau, Fujian Agriculture and Forestry University. The main drafters of this standard. Wangnian Wu, Huang gang water, Chen Jinsong, Shen Jianguo, Wang Hongyi, Wengrui Quan, Huang, Hufang Ping. Mango bacterial black spot disease rapid tests

1 Scope

This standard specifies the mango bacterial black spot pathogen Xanthomonascampestrispv.mangiferaeindicae quarantine identified method. This standard applies to the entry and exit of the mango fruit, leaves, branches, organizations may carry propagation material mango bacterial black spot pathogen detection Phytophthora identification.

2 mango bacterial black spot pathogen basic information

Name. Xanthomonascampestrispv.mangiferaeindicae Taxonomic position. Proteobacteria door (Proteobacteria), γ- Proteobacteria Gang (Gammaproteobacteria), Xanthomonas mesh (Xan- thomonodales), Xanthomonas Branch (Xanthomonodaceae), Xanthomonas (Xanthomonas), black rot Xanthomonas (Xan- thomonascampestris), black rot Xanthomonas campestris pv mango (Xanthomonascampestrispv.mangiferaeindicae) Transmission. long-distance spread mainly by contaminated seedlings, scion and fruits transported. For additional information, see Appendix A.

3 PRINCIPLE OF THE METHOD

According disease genes hrpB mango bacterial black spot pathogen-specific nucleotide sequence to design primers for PCR amplification, and combined with the semi-selected Characters of the culture medium for bacteria quarantine identified.

4 Equipment and Reagents

4.1 Equipment PCR instrument, autoclaves, ice maker, high-speed refrigerated centrifuge, centrifuges, ultra-low temperature freezer, a conventional refrigerator, whirlpool oscillator, electrophoresis Instrument, gel imaging system, the balance (the amount of sense. 0.001g), high-temperature oven, shaker-incubator, biological safety cabinets, adjustable pipette. 4.2 Reagents Semi-selective medium NCTM3 reagent and NA media see Appendix B, PCR detection reagent in Appendix C.

5 Detection Methods

5.1 Symptom Check Carefully observe the mango fruit, leaves and branches, the symptoms described in Appendix A. Collection and sample preparation 5.2 quarantine Take suspected symptoms of mango fruits, leaves, branches and other laboratory testing identified.

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